Umeda M, Yokoo S, Komori T, Nishimatsu N, Shibuya Y, Fujioka M
Department of Oral and Maxillofacial Surgery, Kobe University School of Medicine, Kobe, Japan.
Br J Oral Maxillofac Surg. 2001 Oct;39(5):376-80. doi: 10.1054/bjom.2000.0590.
Three squamous cell carcinoma (SCC) cell lines established from oral cancer, seven specimens of SCC and three of adenoid cystic carcinomas taken from the oral cavity during operations were transplanted into the tongues of nude mice. Metastases to the regional lymph nodes and the lungs were examined histologically. We were able to transplant every cell line or specimen of tissue into the tongue of nude mice, and found that cancer transplanted in the tongue invaded diffusely to the surrounding tissues without forming a capsule, and that the mode of invasion of the transplanted SCC was similar to that of the biopsy specimen of the patient from whom the material had been obtained. We also found that all three of the SCC cell lines, 3 of the 7 SCC specimens and 2 of the 3 adenoid cystic carcinoma tissues metastasized to the regional lymph node. SCC did not metastasize to the lung, but in two of the three adenoid cystic carcinomas we did see micrometastases to the lung. The study indicates that this method can be used as a model of metastasis in oral squamous cell carcinoma and adenoid cystic carcinoma to show the stages of metastasis in cancer.
将3种源自口腔癌的鳞状细胞癌（SCC）细胞系、7份SCC标本以及3份手术中取自口腔的腺样囊性癌标本移植到裸鼠舌部。对区域淋巴结和肺部的转移情况进行组织学检查。我们能够将每个细胞系或组织标本移植到裸鼠舌部，发现移植到舌部的癌症呈弥漫性浸润周围组织，无包膜形成，且移植的SCC的浸润方式与获取该材料的患者活检标本相似。我们还发现，所有3种SCC细胞系、7份SCC标本中的3份以及3份腺样囊性癌组织中的2份转移至区域淋巴结。SCC未转移至肺部，但在3份腺样囊性癌中的2份中，我们确实观察到了肺部微转移。该研究表明，这种方法可作为口腔鳞状细胞癌和腺样囊性癌转移模型，以展示癌症转移的各个阶段。
