外侧下丘脑参与介导扣带回皮质胆碱能刺激引起的降压反应的通路。

The lateral hypothalamus is involved in the pathway mediating the hypotensive response to cingulate cortex-cholinergic stimulation.

作者信息

Pajolla G P, Crippa G E, Corrêa S A, Moreira K B, Tavares R F, Corrêa F M

机构信息

Department of Pharmacology, School of Medicine of Ribeirão Preto, University of São Paulo, Brazil.

出版信息

Cell Mol Neurobiol. 2001 Aug;21(4):341-56. doi: 10.1023/a:1012650021137.

DOI:10.1023/a:1012650021137

PMID:11775065

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11533853/

Abstract

The injection of acetylcholine (ACh) into the medial prefrontal cortex (MPFC) caused marked hypotensive response in either unanesthetized or anesthetized rats. 2. The present experiment was designed to investigate anatomical connections of the ACh injection site in the MPFC with putative autonomic-related brain nuclei, as well as their possible involvement in the mediation of the hypotensive response to ACh. 3. For the above purpose, the bidirectional neuronal tracer biotinylated dextran amine (BDA) was injected into Cg1 and Cg3 areas, within the MPFC of male Wistar rats. Five days later the animals were sacrificed and brain slices were processed and analyzed to determine neuronal projections efferent from as well afferent to the MPFC. 4. Neuronal staining was more prominent in regions ipsilateral to the BDA injection site. Prominent efferent projections of the MPFC were observed in the contralateral MPFC: ipsi- and contralateral amygdala and hypothalamus; ipsilateral septal area, diagonal band, and zona incerta. 5. Similar but not equal patterns of neuronal labeling were observed when BDA injections were performed within the two adjacent MPFC areas. BDA injections centered in the ACh injection site in the Cg3 area caused strong labeling in the septal area and diagonal band as well as an overall hypothalamic labeling. Within the hypothalamus an intense cortical projection was observed in the lateral hypothalamus (LH). BDA injections into the Cg1 area caused a more evident labeling of the amygdaloid complex. 6. Neuronal cell bodies were evident throughout the MPFC as well as in the sensory-motor cortex when BDA was injected into the LH, thus indicating a massive ipsilateral cortical projection from the Cg3 to the LH. 7. Bilateral NMDA-induced lesions within the LH caused a significant attenuation of the depressor responses to ACh injection in the MPFC, whereas unilateral lesions were marginally effective. These results indicate the involvement of the LH in the mediation of the hypotensive response to ACh injection into the MPFC as well as the bilateral distribution of the hypotensive pathway.

摘要

向内侧前额叶皮质（MPFC）注射乙酰胆碱（ACh），在未麻醉或麻醉的大鼠中均引起明显的降压反应。2. 本实验旨在研究MPFC中ACh注射部位与假定的自主神经相关脑核的解剖连接，以及它们在介导对ACh的降压反应中的可能作用。3. 为了上述目的，将双向神经元示踪剂生物素化葡聚糖胺（BDA）注射到雄性Wistar大鼠MPFC内的Cg1和Cg3区域。五天后处死动物，处理并分析脑切片，以确定从MPFC传出和传入的神经元投射。4. 神经元染色在BDA注射部位同侧的区域更为明显。在对侧MPFC、同侧和对侧杏仁核及下丘脑、同侧隔区、斜角带和未定带回观察到MPFC明显的传出投射。5. 当在两个相邻的MPFC区域内进行BDA注射时，观察到相似但不完全相同的神经元标记模式。以Cg3区域的ACh注射部位为中心进行BDA注射，在隔区和斜角带以及整个下丘脑标记中引起强烈标记。在下丘脑内，观察到外侧下丘脑（LH）有强烈的皮质投射。向Cg1区域注射BDA导致杏仁复合体的标记更明显。6. 当将BDA注射到LH中时，整个MPFC以及感觉运动皮质中都有明显的神经元细胞体，因此表明从Cg3到LH有大量同侧皮质投射。7. LH内的双侧NMDA诱导损伤导致对MPFC中ACh注射的降压反应显著减弱，而单侧损伤的效果不明显。这些结果表明LH参与介导对MPFC中ACh注射的降压反应以及降压途径的双侧分布。