Shao Z, Shen Z
Department of Surgery, Molecular Biology Laborafory, Cancer Hospital, Shanghai Medical University, Shanghai 200032.
Zhonghua Zhong Liu Za Zhi. 1999 Sep;21(5):325-8.
To study the mecnanism of inhibition of breast carcinoma cells by genistein.
Human breast cancer cell lines were treated in vitro with genistein. Northern blot, Western blot, plasmid transfection and apoptosis assay were used to evaluate the mechanism of cell growth inhibition.
Genistein could inhibit proliferation of human breast carcinoma cells with different ER status and p53 status. This inhibition was associated with induction of p21WAF1/CIP1 mRNA and protein expression. Exposure of MCF-7 and MDA-MB-231 cells to genistein resulted in statistically significant increased p21WAF1/CIP1 mRNA stability, accompanied with increased p21WAF1/CIP1 protein expression. This increase in p21WAF1/CIP1 was subsequently followed by the onset of apoptosis.
The inhibitory effect of genistein on cell proliferation of breast cancer appears to be due to p21WAF1/CIP1 expression and apoptosis through mechanism independent of ER and p53. The results provide evidence for the potential usefulness of genistein in the prevention and treatment of human breast cancer.
研究染料木黄酮对乳腺癌细胞的抑制机制。
用染料木黄酮体外处理人乳腺癌细胞系。采用Northern印迹法、Western印迹法、质粒转染和凋亡检测来评估细胞生长抑制机制。
染料木黄酮可抑制不同雌激素受体（ER）状态和p53状态的人乳腺癌细胞增殖。这种抑制与p21WAF1/CIP1 mRNA和蛋白表达的诱导有关。MCF-7和MDA-MB-231细胞暴露于染料木黄酮后，p21WAF1/CIP1 mRNA稳定性在统计学上显著增加，同时p21WAF1/CIP1蛋白表达增加。p21WAF1/CIP1的这种增加随后伴随着细胞凋亡的发生。
染料木黄酮对乳腺癌细胞增殖的抑制作用似乎是由于通过独立于ER和p53的机制表达p21WAF1/CIP1并诱导凋亡。这些结果为染料木黄酮在预防和治疗人类乳腺癌中的潜在用途提供了证据。
