He Fu-jin, Wang Jian, Niu Jian-zhao, Wang Ji-feng
Lab. of Cell and Biochemystry, Beijing University of Chinese Meducine, Beijing 100029, China.
Zhongguo Zhong Yao Za Zhi. 2002 Dec;27(12):936-9.
To study the inhibitig effects of genistein on the growth of human breast cancer cell lines in vitro and its mechanisms. METHORD: Human breast cancer cell lines both MCF-7(positive estrogen receptor, ER+) and MDA-MB-231 (negative estrogen receptor, ER-) were cultured in vitro. The proliferation of cells was measured with MTT methord and the growth curve was drawn with cell count. The estrogen receptor in cells was show with immunohistochemistry.
Genistein inhibited proliferation of MCF-7 and MDA-MB-231 cell lines and inhibition was dependent on dose within some concentration range, IC50 being 32.5 mumol.L-1 and 46.8 mumol.L-1 respectively. Genisteins antiproliferation of MCF-7 was stimulated by ectogenesis estrogen but proliferation of MDA-MB-231 inhibited by geinstein was not related to estrogen. The positive signs of ER in cellular nuclei of MCF-7 cell line fed with genistein at concentration of 30 mumol.L-1 were significantly weaker than these not fed with genistein.
Genistein obviously inhibits proliferation of both cell lines of MCF-7 and MDA-MB-231 in vitro and is dependent on dose. Genisteins antiproliferous effect on MCF-7 cell lines is stimulated by estrogen and this effect is related with ER, but genisteins inhibiting proliferation of MDA-MB-231 cell line is not through ER.
研究染料木黄酮对人乳腺癌细胞系体外生长的抑制作用及其机制。方法：体外培养人乳腺癌细胞系MCF-7（雌激素受体阳性，ER+）和MDA-MB-231（雌激素受体阴性，ER-）。采用MTT法检测细胞增殖情况，用细胞计数绘制生长曲线。用免疫组织化学法显示细胞中的雌激素受体。
染料木黄酮在一定浓度范围内抑制MCF-7和MDA-MB-231细胞系的增殖，且抑制作用呈剂量依赖性，IC50分别为32.5μmol·L-1和46.8μmol·L-1。外源性雌激素可刺激染料木黄酮对MCF-7的抗增殖作用，但染料木黄酮对MDA-MB-231增殖的抑制作用与雌激素无关。用30μmol·L-1染料木黄酮处理的MCF-7细胞系细胞核中ER阳性信号明显弱于未用染料木黄酮处理的细胞。
染料木黄酮明显抑制MCF-7和MDA-MB-231两种细胞系的体外增殖，且呈剂量依赖性。染料木黄酮对MCF-7细胞系的抗增殖作用受雌激素刺激，且该作用与ER有关，但染料木黄酮抑制MDA-MB-231细胞系增殖不是通过ER途径。
