Wang A, Huang L, Chen Y
Respiratory Department, Affiliated Hospital of Bengbu Medical College, Bengbu 233004, China.
Zhonghua Jie He He Hu Xi Za Zhi. 2000 Aug;23(8):475-7.
To explore the diagnostic value of telomerase activity for lung cancer.
Telomerase activity was measured by PCR-TRAP in cancerous lesions and contralateral bronchial tissues from patients with lung cancer and in the bronchial tissues from patients with noncancerous pulmonary disorders, in which the tissue specimens were obtained by fiberoptic bronchoscopy. The result of telomerase detection was compared with the positive rates of pathologic study and bronchial brush cytology.
The positive rate of telomerase activity in lesions of lung cancer was significantly higher than that in tissues of the contralateral bronchi from patients of lung cancer (P < 0.01) and that in tissues from patients of noncancerous pulmonary disorders (P < 0.01). There was no significant difference between the positive rate of telomerase activity expression and the positive rates of pathology and bronchial brush cytology (P > 0.05).
Examination of telomerase activity was of significance in the diagnosis of lung cancer.
探讨端粒酶活性检测对肺癌的诊断价值。
采用PCR-TRAP法检测肺癌患者癌灶组织、癌灶对侧支气管组织以及非癌性肺疾病患者支气管组织中的端粒酶活性，组织标本通过纤维支气管镜获取。将端粒酶检测结果与病理检查及支气管刷检细胞学检查的阳性率进行比较。
肺癌病灶中端粒酶活性阳性率显著高于肺癌患者对侧支气管组织（P<0.01）及非癌性肺疾病患者组织（P<0.01）。端粒酶活性表达阳性率与病理及支气管刷检细胞学阳性率之间差异无统计学意义（P>0.05）。
端粒酶活性检测对肺癌诊断具有重要意义。
