晚期肺癌活检组织中的端粒酶及其组分以及痰液中的端粒酶活性

Telomerase activity in sputum and telomerase and its components in biopsies of advanced lung cancer.

作者信息

Pasrija Teena, Srinivasan Radhika, Behera Digambar, Majumdar Siddhartha

机构信息

Department of Pulmonary Medicine, Postgraduate Institute of Medical Education and Research, Education and Research, Sector 12, Chandigarh 160 012, India.

出版信息

Eur J Cancer. 2007 Jun;43(9):1476-82. doi: 10.1016/j.ejca.2007.04.003. Epub 2007 May 24.

DOI:10.1016/j.ejca.2007.04.003

PMID:17531468

Abstract

PURPOSE

In this study, we explored the diagnostic utility of sputum telomerase activity as a non-invasive biomarker of lung cancer. In biopsies of lung cancer, the relationship of telomerase activity to telomerase reverse transcriptase (hTERT) and telomerase RNA component (hTERC) and to c-Myc expression was also evaluated.

METHODS

Paired biopsy and sputum samples were evaluated for telomerase activity by the telomerase repeat amplification protocol (TRAP) assay in 34 cases of lung cancer and in 30 control subjects without any evidence of lung cancer. hTERT and hTERC transcript expression was evaluated in 42 cases of lung cancer and compared to telomerase activity and c-Myc transcript expression.

RESULTS

Telomerase activity was present in 85.2% of biopsies and in 67.6% of paired sputum with a good concordance. Three out of the 30 negative controls showed a weak telomerase activity, all of whom had sarcoidosis. Thus, sputum telomerase activity had sensitivity, specificity, Negative Predictive Value and Positive Predictive Value of 67.6%, 90%, 71% and 88.46%, respectively. The hTERT levels correlated to the telomerase activity but not to the c-Myc oncogene expression.

CONCLUSIONS

In lung cancer, sputum telomerase activity is a candidate non-invasive biomarker of malignancy.

摘要

目的

在本研究中，我们探讨了痰液端粒酶活性作为肺癌非侵入性生物标志物的诊断效用。在肺癌活检中，还评估了端粒酶活性与端粒酶逆转录酶（hTERT）、端粒酶RNA组分（hTERC）以及与c-Myc表达之间的关系。

方法

通过端粒酶重复序列扩增法（TRAP）检测，对34例肺癌患者和30例无肺癌证据的对照受试者的配对活检和痰液样本进行端粒酶活性评估。对42例肺癌患者的hTERT和hTERC转录表达进行评估，并与端粒酶活性和c-Myc转录表达进行比较。

结果

85.2%的活检样本和67.6%的配对痰液样本中端粒酶活性呈阳性，二者一致性良好。30例阴性对照中有3例显示出微弱的端粒酶活性，这3例均患有结节病。因此，痰液端粒酶活性的敏感性、特异性、阴性预测值和阳性预测值分别为67.6%、90%、71%和88.46%。hTERT水平与端粒酶活性相关，但与c-Myc癌基因表达无关。