Nakanishi T, Oka T, Akagi T
Department of Biochemistry and Molecular Dentistry, and Okayama University Graduate School of Medicine and Dentistry, Japan.
Acta Med Okayama. 2001 Dec;55(6):319-28. doi: 10.18926/AMO/32002.
The structure of the human genome is almost completely elucidated and the life sciences will now aim for a general and integrated study of gene expressions and the functional elucidation of proteins. In such a study, various new techniques have been developed, and DNA microarray technology is the most representative one. As for the DNA microarray techniques, several thousands to tens of thousands of gene segments are immobilized on a glass slide at high density, and cDNA probes prepared from specific cells or tissues are hybridized on the slides from which gene expression profiles are obtained at one sweep in a short time. The present development of this technique and its possible application to medicine-related fields are described.
人类基因组的结构几乎已完全阐明，生命科学现在将致力于对基因表达进行全面综合研究以及对蛋白质功能进行阐释。在这样的研究中，已经开发出了各种新技术，而DNA微阵列技术是最具代表性的一种。至于DNA微阵列技术，数千到数万个基因片段被高密度固定在载玻片上，从特定细胞或组织制备的cDNA探针与载玻片杂交，从而在短时间内一次性获得基因表达谱。本文描述了该技术的当前发展及其在医学相关领域的可能应用。
