Zhang X, Sobue T, Hurley M M
University of Connecticut School of Medicine, Farmington, CT 06030, USA.
Biochem Biophys Res Commun. 2002 Jan 11;290(1):526-31. doi: 10.1006/bbrc.2001.6217.
FGF-2 stimulates bone formation in vitro and in vivo in rats. However, there are limited studies in mice and no data on the mechanism(s) by which FGF-2 induces bone formation. We assessed whether short-term FGF-2 treatment of marrow stromal cells from young mice would increase alkaline phosphatase-positive (ALP), mineralized colony formation and expression of genes important in osteoblast maturation. Short-term treatment with FGF-2 (0.01-1.0 nM) for the first 3 days of a 14- or 21-day culture period increased the number of ALP mineralized colonies in bone marrow stromal cells. FGF-2 (0.1 nM) increased the mRNAs for type 1 collagen: osteocalcin, runt domain/core binding factor, PTH/PTHR receptor, and insulin-like growth factor 1 (IGF-1) at 14 and 21 days. We conclude that short-term FGF-2 treatment enhances osteoblast maturation in vitro. Furthermore, the anabolic effect of FGF-2 may be attributed in part to regulation of IGF-1 in osteoblasts.
成纤维细胞生长因子-2（FGF-2）在体外和大鼠体内均能刺激骨形成。然而，关于小鼠的相关研究有限，且尚无关于FGF-2诱导骨形成机制的数据。我们评估了对幼鼠骨髓基质细胞进行短期FGF-2处理是否会增加碱性磷酸酶阳性（ALP）、矿化集落形成以及成骨细胞成熟过程中重要基因的表达。在为期14天或21天的培养期的前3天，用FGF-2（0.01 - 1.0 nM）进行短期处理可增加骨髓基质细胞中ALP矿化集落的数量。在第14天和第21天，FGF-2（0.1 nM）可增加I型胶原、骨钙素、 runt结构域/核心结合因子、甲状旁腺激素/甲状旁腺激素受体以及胰岛素样生长因子1（IGF-1）的mRNA水平。我们得出结论，短期FGF-2处理可在体外增强成骨细胞成熟。此外，FGF-2的合成代谢作用可能部分归因于其对成骨细胞中IGF-1的调节。
