Goh E K, Lee B J, Wang S G, Chon K M, Kim C M
Department of Otolaryngology, College of Medicine, Pusan National University, Pusan Korea.
J Laryngol Otol. 2001 Nov;115(11):881-4. doi: 10.1258/0022215011909495.
Telomerase maintains the length of telomeres in immortal cells and is also often associated with cell proliferation. Cholesteatoma epithelium is characterized by a dysregulation with hyperproliferative growth. The study evaluated the telomerase activity in cholesteatoma and normal retro-auricular skin to discover the relationship between telomerase expression and clinical findings. Twenty-two samples of cholesteatoma and 15 samples of retro-auricular skin were obtained from patients undergoing middle-ear surgery. The telomerase activity was detected by the telomerase repeat amplification protocol (TRAP) assay method. Seventeen of the 22 (77.3 per cent) cholesteatoma cases expressed telomerase activity, whereas none of the 15 retro-auricular normal skin (0 per cent) detected telomerase activity. There was no significant difference between telomerase expressions and clinical findings, including hearing level, duration of disease, and the degree of extension (p>0.05). The high expression of telomerase in cholesteatoma suggests that the activation of telomerase may be related to the proliferative nature of cholesteatoma.
端粒酶维持永生化细胞中端粒的长度，并且通常也与细胞增殖相关。胆脂瘤上皮的特征是生长失调且过度增殖。本研究评估了胆脂瘤和正常耳后皮肤中的端粒酶活性，以发现端粒酶表达与临床结果之间的关系。从接受中耳手术的患者中获取了22份胆脂瘤样本和15份耳后皮肤样本。通过端粒酶重复序列扩增法（TRAP）检测端粒酶活性。22例胆脂瘤病例中有17例（77.3%）表达端粒酶活性，而15份耳后正常皮肤样本中无一例（0%）检测到端粒酶活性。端粒酶表达与临床结果（包括听力水平、病程和扩展程度）之间无显著差异（p>0.05）。胆脂瘤中端粒酶的高表达表明，端粒酶的激活可能与胆脂瘤的增殖特性有关。
