[中耳胆脂瘤中基质金属蛋白酶2和基质金属蛋白酶9活性的检测]

[Detection of the activity of MMP2 and MMP9 in middle ear cholesteatoma].

作者信息

Wang Huibing, Xu Zhiwen, Tang Anzhou, Su Jiping

机构信息

Department of Otolaryngology, First Affiliated Hospital of Guangxi University of Medical Sciences, Nanning, 530021, China.

出版信息

Lin Chuang Er Bi Yan Hou Ke Za Zhi. 2004 Oct;18(10):620-2.

PMID:15620146

Abstract

OBJECTIVE

To study the relationship between the activity of MMP2, and bone resorption in middle ear cholesteatoma.

METHOD

Specimens from 41 cases of middle ear cholesteatoma and 20 cases of external auditory canal skin were analysed for molecular corresponding to MMP2 and MMP9 by using Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) Zymography.

RESULT

The active levels of MMP2 and MMP9 in the cholesteatoma, which were (0.954+/-0.411) and (2.676+/-0.734) respectively, were obviously higher than that in the external auditory canal skins, which were (0.355+/-0.160) and (1.166+/-0.443) gray area x mg(-1) x ml(-1) respectively, and showed close relationship (P < 0.01). The active levels of MMP2 and MMP9 in extensive cholesteatoma, which were (1.062+/-0.401) and (2.946+/-0.134) respectively, were significantly increased in comparison with in localized cholesteatoma which were (0.701+/-0.318) and (2.193+/-0.601) gray area x mg(-1) x ml(-1) respectively. No significant difference was observed between recurrent cases and first cases (P > 0.05).

CONCLUSION

The increase of activity of MMP2 and MMP9 in cholesteatoma indicated that MMP2 and MMP9 may play a vital role in the bone destruction associated with cholesteatoma.

摘要

目的

研究基质金属蛋白酶2（MMP2）活性与中耳胆脂瘤骨吸收之间的关系。

方法

采用十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）酶谱法分析41例中耳胆脂瘤标本和20例外耳道皮肤标本中与MMP2和MMP9相对应的分子。

结果

胆脂瘤中MMP2和MMP9的活性水平分别为（0.954±0.411）和（2.676±0.734），明显高于外耳道皮肤中的活性水平，后者分别为（0.355±0.160）和（1.166±0.443）灰度区x mg⁻¹x ml⁻¹，且两者呈密切关系（P<0.01）。广泛型胆脂瘤中MMP2和MMP9的活性水平分别为（1.062±0.401）和（2.946±0.134），与局限型胆脂瘤中分别为（0.701±0.318）和（2.193±0.601）灰度区x mg⁻¹x ml⁻¹相比，显著升高。复发病例与初发病例之间未观察到显著差异（P>0.05）。