Xie Z, Cao X, Zhang W, Ye X, Yu B, Zheng Z
Department of Cell Biology, Second Military Medical University, Shanghai 200433, China.
Chin Med J (Engl). 2001 Jan;114(1):93-6.
To compare the endocytic routes of exogenous antigen between murine dendritic cells (DCs) and macrophages (M phi s).
Murine bone marrow-derived DCs and peritoneal M phi s were pulsed with horseradish peroxidase (HRP)-5 nm colloidal gold for 10 minutes, then grouped and chased for 0-120 minutes in culture medium. Intracellular distribution of 5 nm colloidal gold was explored by means of the cellular enzymatic-chemistry of acid phosphatase and MHC II immuno-cytochemistry under electron microscope.
After 10 minutes of pulse with HRP-5 nm colloidal gold and 30 minutes of chase, most HRP-5 nm gold particles internalized by DCs entered into MHC class II compartments (M II Cs), and a small portion entered into acid phosphatase-positive lysosomes. In contrast to DCs, most M phi s lysosomes were accessed by HRP-5 nm gold particles, and a small portion of HRP-5 nm gold particles entered into M II Cs. After 60 minutes of chase, 5 nm gold particles could hardly be seen within M phi s, whereas most 5 nm gold particles were still retained in DCs.
The endocytic route of exogenous antigen in DCs seems to be different from that in M phi s. Antigens taken by M phi s mainly enter into lysosomes within 30 minutes. In the case of DCs, most internalized antigens enter into M II Cs, which may be related to their unique antigen-presenting function. In addition, M phi s seem to have more powerful capacity to scavenge exogenous antigen than DCs.
比较小鼠树突状细胞（DCs）和巨噬细胞（Mφs）对外源性抗原的内吞途径。
用辣根过氧化物酶（HRP）-5纳米胶体金脉冲处理小鼠骨髓来源的DCs和腹腔Mφs 10分钟，然后分组并在培养基中追踪0-120分钟。通过电子显微镜下的酸性磷酸酶细胞酶化学和MHC II免疫细胞化学研究5纳米胶体金的细胞内分布。
用HRP-5纳米胶体金脉冲处理10分钟并追踪30分钟后，DCs内化的大多数HRP-5纳米金颗粒进入MHC II类区室（M II Cs），一小部分进入酸性磷酸酶阳性溶酶体。与DCs不同，大多数Mφs的溶酶体被HRP-5纳米金颗粒进入，一小部分HRP-5纳米金颗粒进入M II Cs。追踪60分钟后，Mφs内几乎看不到5纳米金颗粒，而大多数5纳米金颗粒仍保留在DCs中。
DCs对外源性抗原的内吞途径似乎与Mφs不同。Mφs摄取的抗原主要在30分钟内进入溶酶体。就DCs而言，大多数内化抗原进入M II Cs，这可能与其独特的抗原呈递功能有关。此外，Mφs对外源性抗原的清除能力似乎比DCs更强。
