Ramalho-Santos João, Terada Yukihiro, Schatten Gerald
Oregon Health Sciences University, Beaverton , Oregon, USA. jramalho2ci.uc.pt
Fertil Steril. 2002 Jan;77(1):159-61. doi: 10.1016/s0015-0282(01)02957-0.
To determine the possible use of the mammalian acrosomal marker vehicle-associated membrane protein (VAMP)/synaptobrevin to detect acrosome abnormalities in human sperm.
Analysis of human sperm after fixation and staining with an anti-VAMP antibody.
An academic research institution.
PATIENT(S): Semen samples from consenting patients who were participating in an infertility treatment program.
INTERVENTION(S): Human sperm samples were fixed, permeabilized with detergent, and examined by immunocytochemistry.
Immunostaining.
RESULT(S): Observation of sperm from patients with no obvious sperm morphological defects revealed normal looking acrosomes, as assessed by VAMP immunostaining. However, severe acrosome malformations were detected in other cases. The observations registered varied from the absence of a fully formed organelle in samples of patients with globozoospermia to abnormal VAMP staining in samples from patients with acrosomal defects.
VAMP/synaptobrevin may be a useful marker for the functional assessment of acrosomal status in human sperm.
确定哺乳动物顶体标记物囊泡相关膜蛋白（VAMP）/突触融合蛋白在检测人类精子顶体异常方面的潜在用途。
用抗VAMP抗体固定和染色后对人类精子进行分析。
一个学术研究机构。
来自参与不孕症治疗项目的自愿患者的精液样本。
人类精子样本经固定、用去污剂通透处理后，进行免疫细胞化学检测。
免疫染色。
对无明显精子形态缺陷患者的精子进行观察发现，通过VAMP免疫染色评估，顶体外观正常。然而，在其他病例中检测到严重的顶体畸形。观察结果各不相同，从圆头精子症患者样本中完全没有形成完整的细胞器，到顶体缺陷患者样本中VAMP染色异常。
VAMP/突触融合蛋白可能是评估人类精子顶体状态功能的有用标记物。
