Reid S A, Copeman D B
Australian Institute of Tropical and Animal Science, James Cook University, Townsville, Queensland 4811, Australia.
Vet Parasitol. 2002 Feb 27;104(1):79-84. doi: 10.1016/s0304-4017(01)00624-0.
Attempts were made to improve the accuracy of an antibody-detection ELISA for the detection of Trypanosoma evansi infection in cattle by improving the method of preparation of the crude antigen used. An IgG-ELISA was performed with five different antigen preparations: crude soluble antigen, soluble and insoluble fractions of crude antigen treated with 0.1% formalin and whole formalin-fixed trypanosomes treated with either trypsin or 2-mercaptoethanol. An IgM-ELISA using crude soluble antigen was also performed. Each ELISA was evaluated using serum from 44 Indonesian cattle infected with T. evansi and 262 uninfected cattle from Australia. There was no significant difference between the sensitivity or specificity of the IgG-ELISA using each of the five antigens. The IgM-ELISA using a crude untreated lysate was significantly less sensitive (p<0.05) than the IgG-ELISA using the same antigen, trypsin-treated antigen or the 0.1% formalin-treated soluble antigen (68, 64 and 64%, respectively). These results show that these modifications to the method of producing crude antigens for the Ab-ELISA does not improve the accuracy of diagnosis of T. evansi infection in cattle.
研究人员试图通过改进所用粗抗原的制备方法，提高用于检测牛伊氏锥虫感染的抗体检测酶联免疫吸附测定（ELISA）的准确性。用五种不同的抗原制剂进行了IgG-ELISA：粗可溶性抗原、经0.1%福尔马林处理的粗抗原的可溶性和不溶性部分，以及用胰蛋白酶或2-巯基乙醇处理的经福尔马林固定的完整锥虫。还用粗可溶性抗原进行了IgM-ELISA。每种ELISA均使用来自44头感染伊氏锥虫的印度尼西亚牛和262头来自澳大利亚的未感染牛的血清进行评估。使用这五种抗原中的每一种进行的IgG-ELISA在敏感性或特异性方面均无显著差异。使用未经处理的粗裂解物进行的IgM-ELISA的敏感性显著低于使用相同抗原、经胰蛋白酶处理的抗原或0.1%福尔马林处理的可溶性抗原进行的IgG-ELISA（分别为68%、64%和64%，p<0.05）。这些结果表明，对用于抗体ELISA的粗抗原生产方法的这些改进并不能提高牛伊氏锥虫感染诊断的准确性。
