Jaimes-Dueñez Jeiczon, Zapata-Zapata Carolina, Triana-Chávez Omar, Mejía-Jaramillo Ana M
Grupo BCEI, Universidad de Antioquia UdeA, Calle 70 No. 52-21, Medellín, Colombia; Grupo GRICA, Universidad Cooperativa de Colombia UCC, Calle 30 No. 33-51, Bucaramanga, Colombia.
Grupo BCEI, Universidad de Antioquia UdeA, Calle 70 No. 52-21, Medellín, Colombia.
Prev Vet Med. 2019 Aug 1;169:104712. doi: 10.1016/j.prevetmed.2019.104712. Epub 2019 Jun 20.
Surra is a zoonotic disease caused by Trypanosoma evansi, affecting the health and production of the livestock significantly. There are several methods to diagnose this disease, which have different principles, sensitivity, and specificity. Among them, the serological techniques using T. evansi as antigen are powerful tools for its epidemiological surveillance. However, they are poorly used due to inefficient in vitro propagation of T. evansi, which requires the use of laboratory animals for antigen production. In the present study, whole cell lysate of T. brucei brucei propagated in vitro was used as an antigen for the detection of anti-T. evansi immunoglobulin G in cattle through an indirect-ELISA. Based on a total of 45 samples from non-infected and 45 samples from T. evansi infected cattle, the sensitivity and specificity were estimated as 100% and 97.7%, respectively. After the validation, serological and molecular surveys were carried out in 710 cattle samples from two endemic Colombian regions (Antioquia and Arauca departments) for T. evansi where molecular prevalences of ˜7.0% were detected through the year and sporadic outbreaks of T. vivax infections have been associated to low prevalence of this species (<1%). A total of 424 (59.7%) samples were positive by indirect-ELISA T. b. brucei, while PCR test for T. evansi and T. vivax, showed 49 (6.9%) and no positive samples, respectively. Interestingly, categories of animals aged>1 year, Bos taurus breed, and those raised under intensive farming system exhibited a higher seroprevalence to T. evansi (P < 0.05). The results displayed a new alternative for antibody detection anti-T. evansi in livestock, using parasites propagated in vitro as antigen, which presents the advantage of higher standardization potential, and avoid the use of live animal for antigen production. A larger availability of this ELISA will generate useful information for a better understanding of the epidemiologic aspects, as well as for the management and control of these diseases in Colombia. However, the ability of the test to detect and/or cross react with T. vivax infections remains to be investigated.
苏拉病是由伊氏锥虫引起的一种人畜共患病，对家畜的健康和生产有显著影响。有几种诊断这种疾病的方法，它们具有不同的原理、敏感性和特异性。其中，以伊氏锥虫为抗原的血清学技术是其流行病学监测的有力工具。然而，由于伊氏锥虫体外繁殖效率低下，需要使用实验动物来生产抗原，这些技术的应用并不广泛。在本研究中，体外繁殖的布氏布氏锥虫全细胞裂解物被用作抗原，通过间接ELISA检测牛血清中抗伊氏锥虫免疫球蛋白G。基于45份未感染牛的样本和45份伊氏锥虫感染牛的样本，敏感性和特异性分别估计为100%和97.7%。验证后，对来自哥伦比亚两个流行地区（安蒂奥基亚省和阿劳卡省）的710份牛样本进行了血清学和分子学调查，以检测伊氏锥虫。通过全年检测，分子流行率约为7.0%，并且间发性的牛巴贝斯虫感染疫情与该物种的低流行率（<1%）有关。共有424份（59.7%）样本通过间接ELISA检测布氏布氏锥虫呈阳性，而伊氏锥虫和牛巴贝斯虫的PCR检测分别显示49份（6.9%）阳性样本和无阳性样本。有趣的是，年龄>1岁的动物、金牛座品种以及集约化养殖系统饲养的动物对伊氏锥虫的血清阳性率更高（P<0.05）。结果显示了一种新的替代方法，即使用体外繁殖的寄生虫作为抗原检测家畜血清中抗伊氏锥虫抗体，该方法具有更高的标准化潜力，并且避免了使用活体动物生产抗原。这种ELISA检测方法的更广泛应用将为更好地了解流行病学情况以及哥伦比亚这些疾病的管理和控制提供有用信息。然而，该检测方法检测和/或与牛巴贝斯虫感染交叉反应的能力仍有待研究。
