Nonaka Nobuhiro, Kitajima Tomoya, Yokobayashi Shihori, Xiao Guoping, Yamamoto Masayuki, Grewal Shiv I S, Watanabe Yoshinori
Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan.
Nat Cell Biol. 2002 Jan;4(1):89-93. doi: 10.1038/ncb739.
Fission yeast centromeres, like those of higher eukaryotes, are composed of repeated DNA structures and associated heterochromatin protein complexes, that have a critical function in the faithful segregation of chromosomes during cell division. Cohesin protein complexes, which are essential for sister-chromatid cohesion and proper chromosome segregation, are enriched at centromeric repeats. We have identified a functional and physical link between heterochromatin and cohesin. We find that the preferential localization of cohesins at the centromeric repeats is dependent on Swi6, a conserved heterochromatin protein that is required for proper kinetochore function. Cohesin is also enriched at the mating-type heterochromatic region in a manner that depends on Swi6 and is required to preserve the genomic integrity of this locus. We provide evidence that a cohesin subunit Psc3 interacts with Swi6 and its mouse homologue HP1. These data define a conserved function of Swi6/HP1 in recruitment of cohesin to heterochromatic regions, promoting the proper segregation of chromosomes.
裂殖酵母的着丝粒与高等真核生物的着丝粒一样，由重复的DNA结构和相关的异染色质蛋白复合物组成，这些复合物在细胞分裂过程中对染色体的准确分离具有关键作用。黏连蛋白复合物对于姐妹染色单体黏连和正确的染色体分离至关重要，在着丝粒重复序列处富集。我们已经确定了异染色质与黏连蛋白之间的功能和物理联系。我们发现黏连蛋白在着丝粒重复序列处的优先定位依赖于Swi6，Swi6是一种保守的异染色质蛋白，是正确的动粒功能所必需的。黏连蛋白也以依赖于Swi6的方式在交配型异染色质区域富集，并且对于维持该位点的基因组完整性是必需的。我们提供证据表明黏连蛋白亚基Psc3与Swi6及其小鼠同源物HP1相互作用。这些数据定义了Swi6/HP1在将黏连蛋白招募到异染色质区域以促进染色体正确分离方面的保守功能。
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