Lu C, Ye R, Yang Q, Yang X, Li H, Li Y
Renal Department, First Affiliated Hospital of Sun Yat-Sen University of Medical Sciences, Guangzhou 510089, China.
Chin Med J (Engl). 2001 May;114(5):477-80.
To observe the influence of glucose on the expression of glucose transporters (GluTs) in peritoneal tissues.
Mesothelial cells (MsCs) from Sprague-Dawley (SD) rats were cultured in medium with glucose 214.4 mmol/L or 75.5 mmol/L. The normal medium with glucose 17.5 mmol/L was used as control. Total RNA was extracted from each sample after 24 hours incubation. Reverse transcript polymerase chain reaction (RT-PCR) was performed with primers corresponding to sodium-glucose transporter (SGIT1) and GluT1-GluT4. mRNA expression of the above GluTs from each sample was measured with quantitative PCR.
GluT1 and GluT2 mRNA can be detected in MsCs from SD rats, while no positive bands can be found specifically for GluT3, GluT4 and SGiT1. Quantitating the amount of PCR products indicated that the levels of GluT1 mRNA in MsCs cultured 24 h in both 214.4 mmol/L glucose and 75.5 mmol/L glucose medium decreased dramatically compared with that in normal medium (P < or = 0.01). While under the same conditions, the levels of GluT2 mRNA in MsCs cultured 24 h in 214.4 mmol/L and 75.5 mmol/L glucose medium both increased significantly (P < 0.01).
GluT1 is strongly expressed in MsCs under normal glucose levels and decreased dramatically under high glucose conditions, while GluT2 expressed at a low level in normal medium and increased greatly after incubation in high glucose conditions. This may play a great role in glucose absorption during peritoneal dialysis and have some connection with ultrafiltration failure due to the alteration of glucose absorption after long-term dialysis.
观察葡萄糖对腹膜组织中葡萄糖转运体（GluTs）表达的影响。
将来自Sprague-Dawley（SD）大鼠的间皮细胞（MsCs）培养于含214.4 mmol/L或75.5 mmol/L葡萄糖的培养基中。以含17.5 mmol/L葡萄糖的正常培养基作为对照。孵育24小时后从每个样本中提取总RNA。使用对应于钠-葡萄糖转运体（SGIT1）和GluT1-GluT4的引物进行逆转录聚合酶链反应（RT-PCR）。用定量PCR测量每个样本中上述GluTs的mRNA表达。
在SD大鼠的MsCs中可检测到GluT1和GluT2 mRNA，而未发现GluT3、GluT,4和SGiT1的特异性阳性条带。对PCR产物量进行定量分析表明，在214.4 mmol/L葡萄糖和75.5 mmol/L葡萄糖培养基中培养24小时的MsCs中，GluT1 mRNA水平与正常培养基相比均显著降低（P≤0.01）。而在相同条件下，在214.4 mmol/L和75.5 mmol/L葡萄糖培养基中培养24小时的MsCs中，GluT2 mRNA水平均显著升高（P<0.01）。
GluT1在正常葡萄糖水平下在MsCs中强烈表达，在高糖条件下显著降低，而GluT2在正常培养基中表达水平较低，在高糖条件下孵育后显著升高。这可能在腹膜透析期间的葡萄糖吸收中起重要作用，并且与长期透析后葡萄糖吸收改变导致的超滤失败有一定关联。
