Li Y, Liu Z, Liu D, Zhang J, Chen Z, Li L
Institute of Nephropathy, Jinling Hospital, Nanjing University School of Medicine, Nanjing 210002, China.
Chin Med J (Engl). 2001 Aug;114(8):824-8.
To evaluate the role of glucose transporter 1 (GLUT1) in the glucose uptake of glomerular mesangial cells.
Cultured human glomerular mesangial cells were used. The expressions of glucose transporter 1 mRNA and protein were detected with reverse transcription-polymerase chain reaction (RT-PCR), flow cytometry and immunofluorescence staining. Glucose uptake was determined with 2-deoxy-[3H]-D-glucose uptake assay.
The expressions of GLUT1 mRNA and proteins were detected in human mesangial cells. Glucose uptake and its kinetics assay showed that GLUT1 is a functional glucose transporter in cultured human mesangial cells, and that its function could be inhibited by the specific inhibitor, Phloretin.
GLUT1 is the predominant glucose transporter in human mesangial cells, which has the kinetic characteristics of high affinity and low capacity for D-glucose. This suggests that in order for mesangial cells to take up excessive quantities of glucose, as in diabetes, changes in glucose transporter expression, translocation or activity may be required.
评估葡萄糖转运蛋白1（GLUT1）在肾小球系膜细胞葡萄糖摄取中的作用。
使用培养的人肾小球系膜细胞。采用逆转录-聚合酶链反应（RT-PCR）、流式细胞术和免疫荧光染色检测葡萄糖转运蛋白1 mRNA和蛋白的表达。用2-脱氧-[3H]-D-葡萄糖摄取试验测定葡萄糖摄取。
在人系膜细胞中检测到GLUT1 mRNA和蛋白的表达。葡萄糖摄取及其动力学分析表明，GLUT1是培养的人系膜细胞中的一种功能性葡萄糖转运蛋白，其功能可被特异性抑制剂根皮素抑制。
GLUT1是人类系膜细胞中主要的葡萄糖转运蛋白，对D-葡萄糖具有高亲和力和低容量的动力学特征。这表明，在糖尿病等情况下，系膜细胞要摄取过量葡萄糖，可能需要葡萄糖转运蛋白的表达、转运或活性发生变化。
