Barami K, Hao H N, Lotoczky G A, Diaz F G, Lyman W D
Department of Neurosurgery, Wayne State University, Detroit, Michigan 48201, USA.
J Neurosurg. 2001 Aug;95(2):308-15. doi: 10.3171/jns.2001.95.2.0308.
The goal of this study was to establish whether transplanted cells derived from fetal human brain can survive in an ischemic lesion.
Sixteen adult male Mongolian gerbils underwent transient bilateral common carotid artery occlusion. One week later, cell suspensions prepared from fetal human brain were injected using stereotactic guidance into the CA1 region of the hippocampus on one side. On the contralateral side injection of the cell suspension medium only was performed. One week after transplantation, the animals were perfusion fixed and their brains were processed for histological studies as well as expression of neuron and glia-specific antigens. Data from ischemic animals were compared with eight nonischemic gerbils that served as sham-operated controls. Last, the in vivo data were correlated with observations made from matching in vitro cultures of the fetal brain cell suspension. The in vivo data indicated that transplanted human fetus-derived brain cells survived in ischemic lesions of gerbil hippocampus after 1 week, provided that the host animal underwent adequate immunosuppression and the transplanted cells were not incorporated into the scar caused by the transplantation procedure. Unlike their in vivo counterparts, after 1 week, most cultured fetal brain cells expressed either neuron- or astrocyte-specific antigens.
This work demonstrates that xenotransplanted fetal human brain cells are able to survive in an ischemic lesion in a rodent model. These data might be useful for future neural transplantation studies of treatments for cerebrovascular ischemia in humans.
本研究的目的是确定源自人类胎儿大脑的移植细胞能否在缺血性损伤中存活。
16只成年雄性蒙古沙鼠接受了短暂性双侧颈总动脉闭塞。一周后，在立体定向引导下，将从人类胎儿大脑制备的细胞悬液注射到一侧海马体的CA1区域。在对侧仅注射细胞悬液培养基。移植一周后，对动物进行灌注固定，并对其大脑进行组织学研究以及神经元和神经胶质细胞特异性抗原的表达研究。将缺血动物的数据与8只作为假手术对照的非缺血沙鼠的数据进行比较。最后，将体内数据与从匹配的胎儿脑细胞悬液体外培养中获得的观察结果相关联。体内数据表明，只要宿主动物接受了充分的免疫抑制，且移植的细胞未融入移植手术造成的瘢痕中，源自人类胎儿的移植脑细胞在沙鼠海马体缺血性损伤中1周后能够存活。与它们在体内的对应物不同，1周后，大多数培养的胎儿脑细胞表达了神经元或星形胶质细胞特异性抗原。
这项工作表明，异种移植的人类胎儿脑细胞能够在啮齿动物模型的缺血性损伤中存活。这些数据可能对未来人类脑血管缺血治疗的神经移植研究有用。
