Takahata T, Katayama Y, Oshima H, Suma T, Koshinaga M
Department of Neurological Surgery, Nihon University School of Medicine, Tokyo, Japan.
Cell Transplant. 1996 Sep-Oct;5(5 Suppl 1):S13-5. doi: 10.1016/0963-6897(96)00031-0.
In a previous study we demonstrated that fetal hippocampal cells, when transplanted into ischemic lesions of the adult rat hippocampus, can survive in large numbers in the host brain and show the innervation of the transplants by cholinergic fibers originated from the host brain. The present study was undertaken in an attempt to elucidate the hypothesis that the fiber connections forming synapses between the transplanted fetal neurons and the host brain play an important role in the survival of the transplanted cells. We transplanted the polymer-encapsulated fetal hippocampal cells prepared from E17-18 rat fetuses into the ischemic lesions in the adult rat hippocampus at which the CA1 pyramidal cells selectively died, and examined both histochemically or immunohistochemically for their survival and the expression of the synaptic vesicle protein, synaptophysin, and dendritic cytoskeltal protein, microtubule-associated protein 2 (MAP 2) within them. In addition, the cholinergic fibers originated from the host brain were examined by acetylcholine esterase (AChE) histochemistry. The results demonstrated that the polymer-encapsulated hippocampal cells could survive in the brain; however, the number of surviving cells markedly decreased following the transplantation, whereas no host-derived cholinergic fibers penetrated the polymer membrane of the capsules following the transplantation. In the cluster of surviving cells, only slight synaptophysin expression and no extensive growth of the dendrites were detected. The present results indicate that the direct contact between the host brain tissue and the transplant play an important role in the survival of such allografted neurons.
在先前的一项研究中，我们证明，将胎鼠海马细胞移植到成年大鼠海马的缺血性损伤部位后，大量细胞可在宿主脑中存活，并显示出源自宿主脑的胆碱能纤维对移植细胞的神经支配。本研究旨在阐明这样一种假说，即移植的胎儿神经元与宿主脑之间形成突触的纤维连接在移植细胞的存活中起重要作用。我们将从E17 - 18大鼠胎儿制备的聚合物包裹的胎鼠海马细胞移植到成年大鼠海马中CA1锥体细胞选择性死亡的缺血性损伤部位，并通过组织化学或免疫组织化学方法检查细胞的存活情况以及其中突触囊泡蛋白、突触素和树突细胞骨架蛋白微管相关蛋白2（MAP 2）的表达。此外，通过乙酰胆碱酯酶（AChE）组织化学检查源自宿主脑的胆碱能纤维。结果表明，聚合物包裹的海马细胞能够在脑中存活；然而，移植后存活细胞的数量显著减少，并且移植后没有宿主来源的胆碱能纤维穿透胶囊的聚合物膜。在存活细胞簇中，仅检测到轻微的突触素表达，未检测到树突的广泛生长。目前的结果表明，宿主脑组织与移植物之间的直接接触在这种同种异体移植神经元的存活中起重要作用。
