脱辅基肌红蛋白与血红素之间重组反应的动力学及机制
The kinetics and mechanism of the recombination reaction between apomyoglobin and haemin.
作者信息
Adams P A
出版信息
Biochem J. 1976 Nov;159(2):371-6. doi: 10.1042/bj1590371.
Abstract
A simple rapid-mixing technique is described which allows the recombination reaction between apomyoglobin and haemin to be studied within 0.3s of the splitting of myoglobin by dilute HCl. Evidence is presented that indicates that the recombination process occurs between folded 'native' apomyoglobin and monomeric haemin. Postulation of a one (or more)-intermediate recombination process, as suggested by other studies, is not necessary to explain the results. The effect, on the kinetics and mechanism of recombination, of the time of exposure to acid pH of the split myoglobin solution was investigated. The effect of temperature on the recombination kinetics was also studied.
摘要
本文描述了一种简单的快速混合技术，该技术能够在稀盐酸裂解肌红蛋白后的0.3秒内研究脱辅基肌红蛋白与血红素之间的重组反应。有证据表明，重组过程发生在折叠的“天然”脱辅基肌红蛋白与单体血红素之间。正如其他研究所暗示的那样，无需假定一个（或多个）中间重组过程来解释这些结果。研究了裂解肌红蛋白溶液暴露于酸性pH值的时间对重组动力学和机制的影响。还研究了温度对重组动力学的影响。
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