脱辅基肌红蛋白与碱性高铁血红素之间重组反应的动力学
The kinetics of the recombination reaction between apomyoglobin and alkaline haematin.
作者信息
Adams P A
出版信息
Biochem J. 1977 Apr 1;163(1):153-8. doi: 10.1042/bj1630153.
Abstract
Apomyoglobin was prepared by an extremely mild modification of the acid/butanone technique, and the kinetics of the recombination reaction between this preparation and alkaline haematin were studied. The recombination has been shown to be precisely second-order and mono-phasic. Rate constants obtained from the study are in good agreement with values obtained previously by an indirect technique not involving separation of haem and apoprotein.
摘要
脱辅基肌红蛋白是通过对酸/丁酮技术进行极其温和的改进制备的，并研究了该制剂与碱性高铁血红素之间重组反应的动力学。重组反应已被证明是精确的二级单相反应。该研究获得的速率常数与先前通过不涉及血红素和脱辅基蛋白分离的间接技术获得的值高度一致。
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