[联合化疗及重组人粒细胞集落刺激因子动员自体外周血干细胞]

[Mobilization of autologous peripheral blood stem cells by combined chemotherapy and rhG-CSF].

作者信息

Shi H, Gu X, Zhu L

机构信息

Department of Hematology, Beijing Hospital, Beijing 100730, China.

出版信息

Zhonghua Zhong Liu Za Zhi. 2001 Mar;23(2):159-61.

PMID:11783025

Abstract

OBJECTIVE

To study the effect of cyclophosphamide (CTX) combination chemotherapy and recombinant human granulocyte colony-stimulating factor (rhG-CSF) on autologous peripheral blood stem cells (APBSC) mobilization.

METHODS

CTX(2.5 +/- 1.0) g/m2 on day 1, VP-16,600-800 mg and/or Ara-C 1.0-2.0 g/m2 on day 2 were injected i.v. rhG-CSF 300 micrograms/d was injected s.c. when the white blood cell (WBC) count reached nadir until one day before APBSC harvest. When the WBC count was over 3.0 x 10(9)/L, peripheral blood mononuclear cell (MNC) collection was performed once per day until the number of MNC collected was > 4 x 10(8)/kg. CFU-GM colony formation and CD34+ cell enumeration were performed.

RESULTS

Twenty cases were studied. The lowest level of WBC was (1.2 +/- 0.8) x 10(9)/L on day 8.5 +/- 1.5 following chemotherapy. rhG-CSF was given from day 9.0 +/- 2.0 and continued for 6.0 +/- 1.0 days. APBSC harvest began on day 12.0 +/- 2.0 and continued for 4.0 +/- 1.0 days. A total of (6.14 +/- 2.34) x 10(8)/kg MNC and (23.25 +/- 30.56) x 10(6)/kg CD34+ cells was procured. Peak level of CD34+ cells was observed 14.6 +/- 1.8 days after chemotherapy and 4.9 +/- 1.6 days after rhG-CSF administration. CFU-GM was (21.68 +/- 15.39) x 10(4)/kg when rhG-CSF was given for 4.9 +/- 1.6 days. No severe toxic reaction was observed. Hematopoietic reconstitution was very well in all patients received APBSC transplantation.

CONCLUSION

CTX combination chemotherapy followed by rhG-CSF was safe and feasible for APBSC mobilization.

摘要

目的

研究环磷酰胺（CTX）联合化疗及重组人粒细胞集落刺激因子（rhG-CSF）对自体外周血干细胞（APBSC）动员的效果。

方法

第1天静脉注射CTX（2.5±1.0）g/m²，第2天静脉注射VP-16 600 - 800mg和/或阿糖胞苷1.0 - 2.0g/m²。当白细胞（WBC）计数达到最低点时，皮下注射rhG-CSF 300μg/d，直至APBSC采集前一天。当WBC计数超过3.0×10⁹/L时，每天进行一次外周血单个核细胞（MNC）采集，直至采集的MNC数量>4×10⁸/kg。进行CFU-GM集落形成和CD34⁺细胞计数。

结果

研究了20例患者。化疗后第8.5±1.5天WBC最低水平为（1.2±0.8）×10⁹/L。从第9.0±2.0天开始给予rhG-CSF并持续6.0±1.0天。APBSC采集于第12.0±2.0天开始并持续4.0±1.0天。共采集到（6.14±2.34）×10⁸/kg MNC和（23.25±30.56）×10⁶/kg CD34⁺细胞。化疗后14.6±1.8天及rhG-CSF给药后4.9±1.6天观察到CD34⁺细胞峰值水平。rhG-CSF给药4.9±1.6天时CFU-GM为（21.68±15.39）×10⁴/kg。未观察到严重毒性反应。所有接受APBSC移植的患者造血重建良好。