[通过化疗及重组粒细胞集落刺激因子（G-CSF）和粒细胞-巨噬细胞集落刺激因子（GM-CSF）动员自体外周血干细胞]

[Mobilization of autologous peripheral blood stem cells by chemotherapy and recombinant granulocyte colony-stimulating factor (G-CSF) and granulocyte-macrophage colony-stimulating factor (GM-CSF)].

作者信息

Cui X, Shao Y, Ren B, Tong Z, Ren X, Li L, Zhang Z, Zhang N, Dai R, Hao X

机构信息

Cancer Institute and Hospital, Tianjin Medical College, Tianjin 300060, China.

出版信息

Zhonghua Xue Ye Xue Za Zhi. 2000 May;21(5):247-9.

PMID:11876989

Abstract

OBJECTIVE

To observe the efficacy of chemotherapy and recombinant granulocyte colony-stimulating factor (G-CSF, Glycosylated) and granulocyte-macrophage colony-stimulating factor (GM-CSF) in autologous peripheral blood stem cells (APBSC) mobilization.

METHODS

The mobilization regimen: CBP 350 mg/m(2) intravenously injected at day 1, Vp16 350 mg/m(2) intravenously injected from day 1 to day 3. G-CSF and GM-CSF 5 microg x kg(-1) x d(-1) each, subcutaneously injected and DXM 5 mg/d intramuscularly injected, from the day of white blood cell (WBC) recovery to (2.4 - 6.4) x 10(9)/L from nadir to the day before the end of APBSC harvesting. APBSC harvesting started when WBC > 20.0 x 10(9)/L and ended when accumulated mononuclear cells (MNC) > 5 x 10(8)/kg. CFU-GM assay and CD(34)(+) cells counting of the APBSC were performed.

RESULTS

Twenty cases underwent APBSC mobilization. APBSC harvest began at day 22.15 +/- 3.66 for two successive days. Accumulated MNC was (5.93 +/- 1.62) x 10(8)/kg, CD(34)(+) cells (23.10 +/- 11.53) x 10(6)/kg and CFU-GM (3.44 +/- 2.85) x 10(5)/kg. No severe toxicity was observed. Hematopoiesis was well reconstituted in 8 patients received single and in 1 patient received double APBSC transplantations.

CONCLUSION

Chemotherapy combined with G-CSF + GM-CSF was a safe and highly effective method for APBSC mobilization.

摘要

目的

观察化疗联合重组人粒细胞集落刺激因子（G-CSF，糖基化）及粒细胞-巨噬细胞集落刺激因子（GM-CSF）用于自体外周血干细胞（APBSC）动员的疗效。

方法

动员方案：第1天静脉注射卡铂（CBP）350mg/m²，第1至3天静脉注射依托泊苷（Vp16）350mg/m²。自白细胞（WBC）从最低点恢复至（2.4 - 6.4）×10⁹/L之日起至APBSC采集结束前一天，皮下注射G-CSF及GM-CSF各5μg·kg⁻¹·d⁻¹，肌肉注射地塞米松（DXM）5mg/d。当WBC＞20.0×10⁹/L时开始采集APBSC，当累积单个核细胞（MNC）＞5×10⁸/kg时结束采集。对采集的APBSC进行CFU-GM检测及CD34⁺细胞计数。

结果

20例患者接受APBSC动员。连续两天于第22.15±3.66天开始采集APBSC。累积MNC为（5.93±1.62）×10⁸/kg，CD34⁺细胞为（23.10±11.53）×10⁶/kg，CFU-GM为（3.44±2.85）×10⁵/kg。未观察到严重毒性反应。8例接受单次及1例接受两次APBSC移植的患者造血功能均良好重建。