阿糖胞苷联合重组人粒细胞集落刺激因子动员自体外周血干细胞

[Mobilization of autologous peripheral blood stem cells by cytosine arabinoside combined with recombinant human granulocyte colony-stimulating factor].

作者信息

Shi Yuankai, Han Xiaohong, He Xiaohui, Yang Jianliang, Liu Peng

机构信息

Department of Medical Oncology, Cancer Institute (Hospital), Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China.

出版信息

Zhonghua Yi Xue Za Zhi. 2002 Apr 10;82(7):462-6.

PMID:12133516

Abstract

OBJECTIVE

To observe the effect of cytosine arabinoside (Ara-C) combined with recombinant human granulocyte colony-stimulating factor (rhG-CSF) on mobilization of autologous peripheral blood stem cells (APBSCs) among malignant lymphoma patients and investigate appropriate dose of Ara-C.

METHODS

Twenty-two patients with malignant lymphoma were randomly divided into two groups: in group A 3 g/m2.d-1of Ara-C was administered by intravenous drip in 2 divided doses for two days with the total dose of 6 g/m(2); in group B 5 g/m(2).d(-1) of Ara-C was administered by intravenous drip in two divided doses for two days with the total dose of 10 g/m(2). Blood routine examination was made every day. rhG-SCF at the dose of 300 microg.body(-1).d(-1) was injected subcutaneously once a day since the next day when the white blood cells reached the nadir until the end of APBCS harvest. APBSC harvest began when WBC >/= 5.0 x 10(9)/L and finished when the accumulated mononuclear cells >/= 5 x 10(5)/kg.

RESULTS

The median of days when nadir of WBC appeared was 7 days in group A and 10 days in group B. The median of absolute neutrophil count (ANC) at ite nadir was 0.9X109/L in group A and 0.2 x 10(9)/L in group B. The median of days when ANC reached its nadir was 9 days in group A and 13 days in group B. The median of dose of rhG-CSF was 4.35 microg.kg(-1).d(-1) in group A and 4.35 microg.kg(-1).d(-1) in group B. The median of day when rhG-SCF administration began was the 11th day in group A and the 12th day in group B. The median of rhG-SCF administration days was 5 days in group A and 6 days in group B. The median of day when APBSC harvest began was the 15th day after Ara-C administration in group A and the 16th day in group B. The median of harvest time was 2 days in both groups. The volume, speed, and time of each apheresis were similar in the two groups. The time consumed and number of APBSCs collected in each harvest and the total number of APBSCs collected were similar in these two groups.

CONCLUSION

Ara-C combined with rhG-CSF is safe and highly effective for APBSC mobilization. 6 g/m(2) is the suitable dose for APBSC mobilization.

摘要

目的

观察阿糖胞苷（Ara-C）联合重组人粒细胞集落刺激因子（rhG-CSF）对恶性淋巴瘤患者自体外周血干细胞（APBSCs）动员的效果，并探讨Ara-C的合适剂量。

方法

22例恶性淋巴瘤患者随机分为两组：A组给予Ara-C 3 g/m²·d⁻¹，分2次静脉滴注，连用2天，总剂量为6 g/m²；B组给予Ara-C 5 g/m²·d⁻¹，分2次静脉滴注，连用2天，总剂量为10 g/m²。每天进行血常规检查。自白细胞降至最低点次日起，rhG-SCF按300 μg·kg⁻¹·d⁻¹皮下注射，每日1次，直至APBCS采集结束。当白细胞≥5.0×10⁹/L时开始采集APBSC，当累积单个核细胞≥5×10⁵/kg时采集结束。

结果

A组白细胞最低点出现时间的中位数为7天，B组为10天。A组最低点时绝对中性粒细胞计数（ANC）的中位数为0.9×10⁹/L，B组为0.2×10⁹/L。A组ANC降至最低点的天数中位数为9天，B组为13天。A组rhG-CSF剂量的中位数为4.35 μg·kg⁻¹·d⁻¹，B组为4.35 μg·kg⁻¹·d⁻¹。A组rhG-SCF开始给药时间中位数为第11天，B组为第12天。A组rhG-SCF给药天数中位数为5天，B组为6天。A组APBSC采集开始时间中位数为Ara-C给药后第15天，B组为第16天。两组采集时间中位数均为2天。两组每次单采的量、速度和时间相似。两组每次采集消耗的时间、采集的APBSCs数量及采集的APBSCs总数相似。