通里攻下中药对多器官功能障碍综合征大鼠肠道屏障的保护作用

[Protective effects of tongli gongxia herbs on gut barrier in rat with multiple organ dysfunction syndrome].

作者信息

Chen H, Wu X, Guan F

机构信息

First Affiliated Hospital, Dalian Medical University, Liaoning (116011).

出版信息

Zhongguo Zhong Xi Yi Jie He Za Zhi. 2000 Feb;20(2):120-2.

PMID:11783313

Abstract

OBJECTIVE

To explore the protective effects of Tongli Gongxia (TLGX) herbs on gut barrier with multiple organ dysfunction syndrome (MODS).

METHODS

The MODS models were induced by intraperitoneal injection of Zymosan A, and Dachengqi decoction (DCQD) was used in treating MODS models. The levels of endotoxin in the peripheral and portal blood, and the contents of diamine oxidase (DAO), xanthine oxidase (XOD), malondialdehyde (MDA), reduced glutathione (GSH) and tumor necrosis factor (TNF), the permeability of intestinal mucosa and enterocytes, and the histopathologic changes were observed.

RESULTS

The gut barrier function in MODS rats were severely damaged. DCQD could significantly reduce the levels of endotoxin in the blood (P < 0.01), the contents of XOD, MDA and TNF in the blood and intestinal tissue were lowered (P < 0.01), but the levels of GSH in blood and intestinal tissue were raised (P < 0.05). Levels of DAO in the intestinal tissue was markedly increased (P < 0.01). DCQD could reduce the permeability of intestinal mucosa and enterocytes, and attenuate the histopathologic changes of intestinal mucosa in MODS rats.

CONCLUSION

TLGX herbs have apparently protective effects on the gut barrier in MODS rats.

摘要

目的

探讨通里攻下（TLGX）中药对多器官功能障碍综合征（MODS）肠道屏障的保护作用。

方法

通过腹腔注射酵母聚糖A诱导MODS模型，采用大承气汤（DCQD）治疗MODS模型。观察外周血和门静脉血中内毒素水平、二胺氧化酶（DAO）、黄嘌呤氧化酶（XOD）、丙二醛（MDA）、还原型谷胱甘肽（GSH）和肿瘤坏死因子（TNF）的含量、肠黏膜和肠上皮细胞的通透性以及组织病理学变化。

结果

MODS大鼠的肠道屏障功能严重受损。DCQD可显著降低血液中内毒素水平（P < 0.01），降低血液和肠道组织中XOD、MDA和TNF的含量（P < 0.01），但提高血液和肠道组织中GSH的水平（P < 0.05）。肠道组织中DAO水平显著升高（P < 0.01）。DCQD可降低MODS大鼠肠黏膜和肠上皮细胞的通透性，减轻肠黏膜的组织病理学变化。