Xie Ming-zheng, Qi Qing-hui, Zhang Sheng-lin, Wei Ming-ming
Department of General Surgery, First Affiliated Hospital, Dalian Medical University, Dalian, Liaoning Province, 116011, China.
Chin J Integr Med. 2015 Aug;21(8):624-9. doi: 10.1007/s11655-015-1978-8. Epub 2015 Apr 29.
To observe the morphological changes in enteric nerve system (ENS) of rats with multiple organ dysfunction syndrome (MODS) treated by Dachengqi Decoction (, DCQD).
Fifty Wistar rats were randomly assigned to the control group, MODS model group and DCQD treated group. The rats in MODS model group and DCQD treated group were injected Escherichia coli (E. coli) suspension into abdominal cavity under sterile condition. The DCQD treated group was gavaged with DCQD 2 days before the E. coli suspension was injected. Twenty-four hours after injection, the proximal segment of intestine was resected and studied by immunohistofluorescence using vesicular acetylcholine transporter, vasoactive intestinal polypeptide (VIP), substance P (SP) and neuronal nitric oxide synthase (nNOS) antibodies. The whole-mount preparations were observed by laser scanning confocal microscope to detect the changes of quantity and fluorescence integral optical density (IOD) value of intestine enteric nerves.
Compared with the control group, the quantity and IOD value of acetylcholine (ACh), VIP, SP and nitric oxide (NO) nerves of intestine in the MODS group were significantly decreased (P<0.01), and the network of enteric nerves was remarkably disrupted. Compared with the MODS group, the quantity and fluorescence IOD value of ACh, VIP, SP and NO nerves in the DCQD group were significantly increased (P<0.01), and the network of enteric nerves was remarkably recovered.
DCQD can protect and repair damage in the network of ACh, SP, NO and VIP nerves in rats with MODS, which may be one of mechanisms involved in promoting gastrointestinal motility by DCQD.
观察大承气汤（DCQD）治疗多器官功能障碍综合征（MODS）大鼠肠神经系统（ENS）的形态学变化。
将50只Wistar大鼠随机分为对照组、MODS模型组和DCQD治疗组。MODS模型组和DCQD治疗组大鼠在无菌条件下腹腔注射大肠杆菌（E. coli）悬液。DCQD治疗组在注射大肠杆菌悬液前2天灌胃给予DCQD。注射后24小时，切除肠近端，采用免疫荧光法，用囊泡乙酰胆碱转运体、血管活性肠肽（VIP）、P物质（SP）和神经元型一氧化氮合酶（nNOS）抗体进行研究。通过激光扫描共聚焦显微镜观察整装标本，检测肠壁内神经数量及荧光积分光密度（IOD）值的变化。
与对照组比较，MODS组肠壁内乙酰胆碱（ACh）、VIP、SP和一氧化氮（NO）能神经的数量及IOD值显著降低（P<0.01），肠神经网络明显破坏。与MODS组比较，DCQD组ACh、VIP、SP和NO能神经的数量及荧光IOD值显著增加（P<0.01），肠神经网络明显恢复。
DCQD可保护和修复MODS大鼠ACh、SP、NO和VIP能神经网络的损伤，这可能是DCQD促进胃肠动力的机制之一。
