Chaubey A, Gerard M, Singh V S, Malhotra B D
Biomolecular Electronics and Conducting Polymer Research Group, National Physical Laboratory, New Delhi, India.
Appl Biochem Biotechnol. 2001 Oct-Dec;96(1-3):293-301.
Tetraethylorthosilicate (TEOS)-derived sol-gel films were utilized for the immobilization of lactate dehydrogenase (LDH) by physical adsorption and sol-gel/LDH/sol-gel sandwich configuration. An attempt was made to ascertain the optimum pH and temperature for the immobilized LDH. It was shown that TEOS-derived sol-gel films containing physically adsorbed LDH exhibited linearity from 0.5 to 4 mM, whereas those containing LDH in sandwich configuration showed linearity from 0.5 to 3 mM L-lactate. These sol-gel films, immobilized with LDH, were found to be stable for about 4 weeks at 4-10 degrees C.
采用正硅酸四乙酯（TEOS）衍生的溶胶-凝胶薄膜，通过物理吸附和溶胶-凝胶/LDH/溶胶-凝胶夹心结构固定乳酸脱氢酶（LDH）。尝试确定固定化LDH的最佳pH值和温度。结果表明，物理吸附LDH的TEOS衍生溶胶-凝胶薄膜在0.5至4 mM范围内呈线性，而夹心结构中含有LDH的薄膜在0.5至3 mM L-乳酸范围内呈线性。这些固定有LDH的溶胶-凝胶薄膜在4-10摄氏度下可稳定约4周。
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