乳酸安培溶胶-凝胶生物传感器在顺序注射法测定L-乳酸中的应用。

Application of lactate amperometric sol-gel biosensor to sequential injection determination of L-lactate.

作者信息

Gomes Sofia Piedade, Odlozilíková Martina, Almeida M Gabriela, Araújo Alberto N, Couto Cristina M C M, Montenegro M Conceição B S M

机构信息

REQUIMTE, Departamento de Química-Física, Faculdade de Farmácia, Universidade do Porto, Rua Anibal Cunha, 164, 4099-030 Porto, Portugal.

出版信息

J Pharm Biomed Anal. 2007 Mar 12;43(4):1376-81. doi: 10.1016/j.jpba.2006.11.027. Epub 2007 Jan 4.

DOI:10.1016/j.jpba.2006.11.027

PMID:17207600

Abstract

This work describes the construction and evaluation of lactate sol-gel biosensors to accomplish the determination of lactate in pharmaceutical products. Lactate oxidase was incorporated in a porous sol-gel film placed onto a platinum-based electrode. Acid and basic catalysis were assessed. When coupled to a sequential injection system (SIA) the biosensor, based on (3-aminopropyl)trimethoxysilane, 2-(3,4-epoxycyclohexyl)ethyl-trimethoxysilane, deionised water, polyethylene glycol 6000 and acid catalyst, presented a range of linearity of 5x10(-5) to 5x10(-3)M. The analytical usefulness of the developed biosensor was evaluated through analysis of commercial pharmaceutical products containing lactate with a sampling rate of 40 samples h(-1). The enzyme remained active for at least 30 days, enabling about 700 determinations without sensitivity decrease.

摘要

这项工作描述了乳酸盐溶胶-凝胶生物传感器的构建与评估，以实现药品中乳酸盐的测定。乳酸氧化酶被纳入置于铂基电极上的多孔溶胶-凝胶薄膜中。评估了酸碱催化作用。当与顺序注射系统（SIA）耦合时，基于（3-氨丙基）三甲氧基硅烷、2-（3,4-环氧环己基）乙基三甲氧基硅烷、去离子水、聚乙二醇6000和酸催化剂的生物传感器呈现出5×10⁻⁵至5×10⁻³M的线性范围。通过对含乳酸的市售药品进行分析，以40个样品/小时的采样率评估了所开发生物传感器的分析实用性。该酶至少保持活性30天，能够进行约700次测定且灵敏度不降低。