Singh Suman, Singhal Rahul, Malhotra B D
Central Mechanical Engineering Research Institute, G. Avenue, Durgapur 713209, West Bengal, India.
Anal Chim Acta. 2007 Jan 23;582(2):335-43. doi: 10.1016/j.aca.2006.09.010. Epub 2006 Sep 16.
Cholesterol oxidase (ChOx) and cholesterol esterase (ChEt) have been covalently immobilized onto tetraethylorthosilicate (TEOS) sol-gel films. The tetraethylorthosilicate sol-gel/ChEt/ChOx enzyme films thus prepared have been characterized using scanning electron microscopic (SEM), UV-vis spectroscopic, Fourier-transform-infrared (FTIR) spectroscopic and amperometric techniques, respectively. The results of photometric measurements carried out on tetraethylorthosilicate sol-gel/ChEt/ChOx reveal thermal stability up to 55 degrees C, response time as 180 s, linearity up to 780 mg dL(-1) (12 mM), shelf life of 1 month, detection limit of 12 mg dL(-1) and sensitivity as 5.4 x 10(-5) Abs. mg(-1) dL(-1).
胆固醇氧化酶（ChOx）和胆固醇酯酶（ChEt）已被共价固定在原硅酸四乙酯（TEOS）溶胶-凝胶薄膜上。通过扫描电子显微镜（SEM）、紫外-可见光谱、傅里叶变换红外（FTIR）光谱和安培技术分别对由此制备的原硅酸四乙酯溶胶-凝胶/ChEt/ChOx酶薄膜进行了表征。对原硅酸四乙酯溶胶-凝胶/ChEt/ChOx进行的光度测量结果显示，其热稳定性高达55摄氏度，响应时间为180秒，线性范围高达780毫克/分升（12毫摩尔），保质期为1个月，检测限为12毫克/分升，灵敏度为5.4×10⁻⁵吸光度·毫克⁻¹·分升⁻¹。
Zotero 插件