Pacini Stefania, Gulisano Massimo, Vannucchi Simonetta, Ruggiero Marco
Department of Anatomy, University of Firenze, viale Morgagni 50, Firenze, I-50134, Italy.
Biochem Biophys Res Commun. 2002 Jan 18;290(2):820-3. doi: 10.1006/bbrc.2001.6254.
The effects of heparin on angiogenesis are controversial, with some studies claiming stimulatory and other studies claiming inhibitory effects. Since heparin in human plasma is complexed with basic peptides and proteins, we studied the angiogenic effect of complexes resulting by mixing poly-L-lysine (a basic heparin-binding polypeptide) and heparin. Angiogenesis was investigate by chorioallantoic membrane assay. In specimens treated with PBS (negative control), or poly-L-lysine, no significant vascular reaction was detectable. Heparin induced only moderate angiogenic response. However, neutral complexes purified from a mixture of poly-L-lysine and heparin (20/1, w/w) induced a very strong angiogenic response. These results demonstrate that the angiogenic effect of heparin was associated with neutralization of electric charge when the polysaccharide was complexed with a basic peptide.
肝素对血管生成的影响存在争议，一些研究称其具有刺激作用，而另一些研究则称其具有抑制作用。由于人血浆中的肝素与碱性肽和蛋白质结合，我们研究了通过混合聚-L-赖氨酸（一种碱性肝素结合多肽）和肝素形成的复合物的血管生成作用。通过绒毛尿囊膜试验研究血管生成。在用PBS（阴性对照）或聚-L-赖氨酸处理的标本中，未检测到明显的血管反应。肝素仅诱导中度血管生成反应。然而，从聚-L-赖氨酸和肝素（20/1，w/w）混合物中纯化的中性复合物诱导了非常强烈的血管生成反应。这些结果表明，当多糖与碱性肽复合时，肝素的血管生成作用与电荷中和有关。
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