Seidlitz Eric, Korbie Darren, Marien Lindsay, Richardson Mary, Singh Gurmit
Juravinski Cancer Centre, Hamilton, Ontario, Canada.
Microvasc Res. 2004 Mar;67(2):105-16. doi: 10.1016/j.mvr.2003.12.005.
We present a method whereby en face estimation of the chorionic capillary plexus can be generated in the living chick chorioallantoic membrane (CAM) and confirmed by post-fixation cross section analysis. This value does not alter significantly with age and provides a reliable and simple method to evaluate anti-angiogenesis. Anti-angiogenesis may be induced by an intervention, such as a pharmacological agent, applied to the surface of the CAM. We describe the use of silastic rings that are associated with minimal inflammatory reaction, in this process. By estimating changes in the chorionic capillary plexus to quantify anti-angiogenesis, together with silastic rings, we examined the anti-angiogenic effect of human angiostatin and demonstrated that although there is a significant loss of capillaries en face after exposure from days 7 to 9 of incubation, in contrast there is no significant inhibition after exposure to a similar dose of angiostatin from days 11 to 13 of incubation. This not only demonstrates the important effects on neo-angiogenesis compared to mature vessels, but also illustrates the potential of the CAM to readily provide a means for such a comparison.
我们提出了一种方法，通过该方法可以在活的鸡胚绒毛尿囊膜（CAM）中对绒毛膜毛细血管丛进行表面估计，并通过固定后横截面分析进行确认。该值不会随年龄显著变化，并提供了一种可靠且简单的评估抗血管生成的方法。抗血管生成可能由应用于CAM表面的干预措施（如药物制剂）诱导。在此过程中，我们描述了使用与最小炎症反应相关的硅橡胶环。通过估计绒毛膜毛细血管丛的变化以量化抗血管生成，并结合硅橡胶环，我们研究了人血管抑素的抗血管生成作用，结果表明，虽然在孵化第7至9天暴露后表面毛细血管有显著损失，但相比之下，在孵化第11至13天暴露于相似剂量的血管抑素后没有显著抑制作用。这不仅证明了与成熟血管相比对新血管生成的重要影响，还说明了CAM有潜力轻松提供进行这种比较的手段。
