Baldrian Petr, Gabriel Jirí
Laboratory of Biochemistry of the Wood-rotting Fungi, Institute of Microbiology, ASCR, Vídenská 1083, 14220 4, Prague, Czech Republic.
FEMS Microbiol Lett. 2002 Jan 2;206(1):69-74. doi: 10.1111/j.1574-6968.2002.tb10988.x.
Addition of copper (0.5-5 mM) or cadmium (1-5 mM) to the white rot fungus Pleurotus ostreatus cultivated in liquid nitrogen-limited medium for 12 days increased the activity of laccase. The addition of 2 mM Cd led to an 18.5-fold increase of activity, 1 mM Cu increased the activity eight-fold. When added earlier than 12 days, the activation of laccase was delayed (Cu) or decreased (Cd). Ag, Hg, Pb, Zn, and H(2)O(2) decreased laccase activity. To study the effect on native enzymes, purified laccase was incubated with Cd, Cu, and Hg. The addition of Hg decreased the activity of laccase immediately and reduced the temporal stability of the enzyme, while the addition of Cu (0.05-50 mM) increased both enzyme activity and stability. Laccase extracted at different stages of straw colonisation differed in its response to heavy metals.
在液氮限制培养基中培养12天的白腐真菌糙皮侧耳中添加铜（0.5 - 5 mM）或镉（1 - 5 mM）可提高漆酶活性。添加2 mM镉导致活性增加18.5倍，1 mM铜使活性增加8倍。若在12天之前添加，漆酶的激活会延迟（铜）或降低（镉）。银、汞、铅、锌和过氧化氢会降低漆酶活性。为研究对天然酶的影响，将纯化的漆酶与镉、铜和汞一起孵育。添加汞会立即降低漆酶活性并降低酶的时间稳定性，而添加铜（0.05 - 50 mM）则会增加酶活性和稳定性。在秸秆定殖不同阶段提取的漆酶对重金属的反应不同。
