Eichlerová I, Homolka L, Nerud F
Institute of Microbiology, Academy of Sciences of the Czech Republic, 142 20 Prague, Czechia.
Folia Microbiol (Praha). 2003;48(6):775-9. doi: 10.1007/BF02931513.
The effect of enhanced laccase (Lac) activity (obtained after copper addition to cultivation media) on decolorization of azo dye Orange G in two basidiospore-derived monokaryotic isolates of Pleurotus ostreatus was determined. The high Lac-producing isolate efficiently decolorized Orange G. The low-producing isolate showed only poor decolorization ability during cultivation in liquid medium and no decolorization on agar plates containing Orange G after a 25-d growth. A substantial enhancement of Lac activity caused by copper addition into cultivation media was detected in both isolates but, at the same time, the biomass production decreased and decolorization rate was reduced.
测定了在两种糙皮侧耳担子孢子衍生的单核分离株中，增强的漆酶（Lac）活性（通过向培养基中添加铜获得）对偶氮染料橙黄G脱色的影响。高产漆酶的分离株能高效地使橙黄G脱色。低产漆酶的分离株在液体培养基培养过程中脱色能力较差，在含橙黄G的琼脂平板上生长25天后未出现脱色现象。在两种分离株中均检测到向培养基中添加铜导致漆酶活性显著增强，但与此同时，生物量产量下降，脱色率降低。
