Mateasik Anton, Sikurová Libusa, Chorvát Dusan
International Laser Center, Ilkovicova 3, Bratislava 812 19, Slovak Republic.
Bioelectrochemistry. 2002 Jan;55(1-2):173-5. doi: 10.1016/s1567-5394(01)00140-2.
In this work, phospholipid liposomes were used to investigate the influence of lipid negative charge on the interaction of merocyanine 540 (MC540) with model membranes. Liposomes were prepared from a mixture of neutral dimyristoyl lecithin (DMPC) and negatively charged dimyristoyl phosphatidic acid (DMPA). A strong dependence between the presence of charges on the membrane and dye association was found. The affinity of the dye to liposomes was decreased with an increasing content of DMPA in liposomes. Changes in absorption spectra of MC540 suggest that the decrease in affinity of MC540 to charged membranes is accompanied by a hypsochromic solvatochromic shift and changes in monomer/dimer equilibrium of MC540 incorporated in the membrane.
在这项工作中，磷脂脂质体被用于研究脂质负电荷对部花青540（MC540）与模型膜相互作用的影响。脂质体由中性的二肉豆蔻酰卵磷脂（DMPC）和带负电荷的二肉豆蔻酰磷脂酸（DMPA）的混合物制备而成。研究发现膜上电荷的存在与染料缔合之间存在强烈的相关性。随着脂质体中DMPA含量的增加，染料对脂质体的亲和力降低。MC540吸收光谱的变化表明，MC540对带电膜亲和力的降低伴随着向紫移的溶剂化显色位移以及膜中MC540单体/二聚体平衡的变化。
