Hoebeke M, Piette J, van de Vorst A
Institute of Physics B5, University of Liege, Belgium.
J Photochem Photobiol B. 1991 Jun;9(3-4):281-94. doi: 10.1016/1011-1344(91)80166-f.
The production of singlet oxygen by merocyanine 540 was studied in dimyristoyl-phosphatidylcholine liposomes using two singlet oxygen probes: 9,10-anthracenedipropionic acid (water soluble) and 9,10-dimethylanthracene (liposoluble). Upper and lower limits of singlet oxygen quantum yield for bound merocyanine 540 were determined to be 0.055 and 0.015 respectively. The diffusion characteristics of singlet oxygen were examined using the isotropic enhancement effect of D2O and the inhibitory effect of sodium azide. It was shown that 1O2 spent more than 87% of its lifetime in a vesicle environment. When the singlet-reacting substrate and the dye were both located in the bilayer, approximately 40% of the singlet oxygen remained in the liposomes where it was originally generated.
9,10-蒽二丙酸（水溶性）和9,10-二甲基蒽（脂溶性），在二肉豆蔻酰磷脂酰胆碱脂质体中研究了部花青540产生单线态氧的情况。结合态部花青540的单线态氧量子产率的上限和下限分别确定为0.055和0.015。利用重水的各向同性增强效应和叠氮化钠的抑制效应研究了单线态氧的扩散特性。结果表明，1O2在囊泡环境中度过了其寿命的87%以上。当单线态反应底物和染料都位于双分子层中时，约40%的单线态氧保留在其最初产生的脂质体中。
