Lois Carlos, Hong Elizabeth J, Pease Shirley, Brown Eric J, Baltimore David
Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA.
Science. 2002 Feb 1;295(5556):868-72. doi: 10.1126/science.1067081. Epub 2002 Jan 10.
Single-cell mouse embryos were infected in vitro with recombinant lentiviral vectors to generate transgenic mice carrying the green fluorescent protein (GFP) gene driven by a ubiquitously expressing promoter. Eighty percent of founder mice carried at least one copy of the transgene, and 90% of these expressed GFP at high levels. Progeny inherited the transgene(s) and displayed green fluorescence. Mice generated using lentiviral vectors with muscle-specific and T lymphocyte-specific promoters expressed high levels of GFP only in the appropriate cell types. We have also generated transgenic rats that express GFP at high levels, suggesting that this technique can be used to produce other transgenic animal species.
将单细胞小鼠胚胎在体外用重组慢病毒载体进行感染，以生成携带由普遍表达启动子驱动的绿色荧光蛋白（GFP）基因的转基因小鼠。80%的奠基小鼠携带至少一个转基因拷贝，其中90%高水平表达GFP。后代继承了转基因并表现出绿色荧光。使用具有肌肉特异性和T淋巴细胞特异性启动子的慢病毒载体生成的小鼠仅在适当的细胞类型中高水平表达GFP。我们还生成了高水平表达GFP的转基因大鼠，这表明该技术可用于产生其他转基因动物物种。
