疟原虫生命周期中多基因家族不同基因库的阶段特异性转录。
Stage-specific transcription of distinct repertoires of a multigene family during Plasmodium life cycle.
作者信息
Preiser P R, Khan S, Costa F T M, Jarra W, Belnoue E, Ogun S, Holder A A, Voza T, Landau I, Snounou G, Rénia L
机构信息
Division of Parasitology, National Institute for Medical Research, The Ridgeway, London, NW7 1AA, UK.
出版信息
Science. 2002 Jan 11;295(5553):342-5. doi: 10.1126/science.1064938.
Members of a multigene family in the rodent malaria parasite Plasmodium yoelii yoelii code for 235-kilodalton proteins (Py235) that are located in the merozoite apical complex, are implicated in virulence, and may determine red blood cell specificity. We show that distinct subsets of py235 genes are expressed in sporozoites and hepatic and erythrocytic stages. Antibodies to Py235 inhibited sporozoite invasion of hepatocytes. The switch in expression profile occurred immediately after transition from one stage to another. The results suggest that this differential expression is driven by strong biological requirements and provide evidence that hepatic and erythrocytic merozoites differ.
啮齿动物疟原虫约氏疟原虫中的一个多基因家族成员编码235千道尔顿蛋白（Py235），这些蛋白位于裂殖子顶端复合体中，与毒力有关，并且可能决定红细胞特异性。我们发现，py235基因的不同亚群在子孢子、肝期和红细胞期表达。针对Py235的抗体抑制了子孢子对肝细胞的侵袭。表达谱的转变在从一个阶段过渡到另一个阶段后立即发生。结果表明，这种差异表达是由强烈的生物学需求驱动的，并提供了肝期和红细胞期裂殖子不同的证据。
Zotero 插件