Department of Molecular Microbiology & Immunology , Johns Hopkins Bloomberg School of Public Health , 615 North Wolfe Street , Baltimore , Maryland 21205 , United States.
Department of Biological Chemistry , Johns Hopkins School of Medicine , 733 N. Broadway , Baltimore , Maryland 21205 , United States.
J Proteome Res. 2019 Sep 6;18(9):3404-3418. doi: 10.1021/acs.jproteome.9b00324. Epub 2019 Jul 23.
The pre-erythrocytic liver stage of the malaria parasite, comprising sporozoites and the liver stages into which they develop, remains one of the least understood parts of the lifecycle, in part owing to the low numbers of parasites. Nonetheless, it is recognized as an important target for antimalarial drugs and vaccines. Here we provide the first proteomic analysis of merosomes, which define the final phase of the liver stage and are responsible for initiating the blood stage of infection. We identify a total of 1879 parasite proteins, and a core set of 1188 proteins quantitatively detected in every biological replicate, providing an extensive picture of the protein repertoire of this stage. This unique data set will allow us to explore key questions about the biology of merosomes and hepatic merozoites.
疟原虫的红细胞前期肝脏阶段，包括孢子和它们发育成的肝脏阶段，仍然是生命周期中最不被了解的部分之一，部分原因是寄生虫数量较少。尽管如此，它还是被认为是抗疟药物和疫苗的重要靶点。在这里，我们首次对定义肝脏阶段最后阶段并负责启动感染血液阶段的 merosomes 进行了蛋白质组学分析。我们总共鉴定了 1879 种寄生虫蛋白，并且在每个生物学重复中都定量检测到了 1188 种核心蛋白，为这个阶段的蛋白质组提供了广泛的图片。这个独特的数据集将使我们能够探索关于 merosomes 和肝裂殖子生物学的关键问题。
