Harvard School of Public Health.
J Infect Dis. 2013 Nov 15;208(10):1679-87. doi: 10.1093/infdis/jit385. Epub 2013 Jul 31.
Plasmodium falciparum is an intracellular protozoan parasite that infects erythrocytes and hepatocytes. The blood stage of its life cycle causes substantial morbidity and mortality associated with millions of infections each year, motivating an intensive search for potential components of a multi-subunit vaccine. In this study, we present data showing that antibodies from natural infections can recognize a recombinant form of the relatively conserved merozoite surface antigen, PfRH5. Furthermore, we performed invasion inhibition assays on clinical isolates and laboratory strains of P. falciparum in the presence of affinity purified antibodies to RH5 and show that these antibodies can inhibit invasion in vitro.
疟原虫是一种感染红细胞和肝细胞的胞内原生动物寄生虫。其生命周期的血液阶段会导致大量的发病率和死亡率，每年与数百万感染相关，这促使人们积极寻找多亚基疫苗的潜在成分。在这项研究中，我们提供的数据表明，来自自然感染的抗体可以识别相对保守的裂殖子表面抗原 PfRH5 的重组形式。此外，我们在 RH5 的亲和纯化抗体存在下对临床分离株和实验室株的疟原虫进行了入侵抑制测定，结果表明这些抗体可以在体外抑制入侵。
