Katona E, Mărgineanu D G, Vasilescu V
Cell Tissue Res. 1979;203(2):331-8. doi: 10.1007/BF00237247.
The kinetics of water replacement with heavy water (deuterium oxide) in the gastrocnemius and sartorius muscles of the frog under isotonic conditions, studied both gravimetrically and by infrared photometry, reveals three water compartments: (i) non-exchangeable (approximately 80 ml/kg fresh weight), (ii) slowly exchanging (approximately 500 ml/kg fresh weight), (iii) rapid exchanging--extracellular (approximately 200 ml/kg fresh weight). Exposure to both glycerol and glutaraldehyde increases the permeability coefficients and the amount of rapid exchanging water; glutaraldehyde also increases the amount of non-exchangeable water. Approximately 90% of the water is kept in the tissue only by weak intermolecular forces, the energies of which amount to 1 kcal/mol. The amount of non-exchangeable water is equivalent to about six continuous adsorption layers covering the myofilaments. Approximately 70% of the tissue water appears to be replaced by glutaraldehyde during standard fixation.
在等渗条件下，通过重量法和红外光度法研究了青蛙腓肠肌和缝匠肌中重水（氧化氘）替代水的动力学，结果显示有三个水室：（i）不可交换水（约80毫升/千克鲜重），（ii）缓慢交换水（约500毫升/千克鲜重），（iii）快速交换水——细胞外水（约200毫升/千克鲜重）。暴露于甘油和戊二醛都会增加渗透系数以及快速交换水的量；戊二醛还会增加不可交换水的量。大约90%的水仅通过弱分子间力保留在组织中，这些力的能量为1千卡/摩尔。不可交换水的量相当于覆盖肌丝的约六个连续吸附层。在标准固定过程中，约70%的组织水似乎被戊二醛替代。
