NADPH 依赖性 L-山梨糖还原酶负责氧化葡萄糖杆菌 IFO 3291 中 L-山梨糖的同化作用。
NADPH-dependent L-sorbose reductase is responsible for L-sorbose assimilation in Gluconobacter suboxydans IFO 3291.
作者信息
Shinjoh Masako, Tazoe Masaaki, Hoshino Tatsuo
机构信息
Department of Applied Microbiology, Nippon Roche Research Center, 200 Kajiwara, Kamakura 247, Japan.
出版信息
J Bacteriol. 2002 Feb;184(3):861-3. doi: 10.1128/JB.184.3.861-863.2002.
Abstract
The NADPH-dependent L-sorbose reductase (SR) of L-sorbose-producing Gluconobacter suboxydans IFO 3291 contributes to intracellular L-sorbose assimilation. The gene disruptant showed no SR activity and did not assimilate the once-produced L-sorbose, indicating that the SR functions mainly as an L-sorbose-reducing enzyme in vivo and not as a D-sorbitol-oxidizing enzyme.
摘要
产L-山梨糖的弱氧化葡糖杆菌IFO 3291中依赖烟酰胺腺嘌呤二核苷酸磷酸（NADPH）的L-山梨糖还原酶（SR）有助于细胞内L-山梨糖的同化作用。该基因破坏突变体没有SR活性，也不能同化曾经产生的L-山梨糖，这表明SR在体内主要作为L-山梨糖还原酶发挥作用，而不是作为D-山梨醇氧化酶发挥作用。
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