铜绿假单胞菌PAO1（一种机会致病菌）的全基因组序列

Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen.

作者信息

Stover C K, Pham X Q, Erwin A L, Mizoguchi S D, Warrener P, Hickey M J, Brinkman F S, Hufnagle W O, Kowalik D J, Lagrou M, Garber R L, Goltry L, Tolentino E, Westbrock-Wadman S, Yuan Y, Brody L L, Coulter S N, Folger K R, Kas A, Larbig K, Lim R, Smith K, Spencer D, Wong G K, Wu Z, Paulsen I T, Reizer J, Saier M H, Hancock R E, Lory S, Olson M V

机构信息

PathoGenesis Corporation, Seattle, Washington 98119, USA.

出版信息

Nature. 2000 Aug 31;406(6799):959-64. doi: 10.1038/35023079.

DOI:10.1038/35023079

PMID:10984043

Abstract

Pseudomonas aeruginosa is a ubiquitous environmental bacterium that is one of the top three causes of opportunistic human infections. A major factor in its prominence as a pathogen is its intrinsic resistance to antibiotics and disinfectants. Here we report the complete sequence of P. aeruginosa strain PAO1. At 6.3 million base pairs, this is the largest bacterial genome sequenced, and the sequence provides insights into the basis of the versatility and intrinsic drug resistance of P. aeruginosa. Consistent with its larger genome size and environmental adaptability, P. aeruginosa contains the highest proportion of regulatory genes observed for a bacterial genome and a large number of genes involved in the catabolism, transport and efflux of organic compounds as well as four potential chemotaxis systems. We propose that the size and complexity of the P. aeruginosa genome reflect an evolutionary adaptation permitting it to thrive in diverse environments and resist the effects of a variety of antimicrobial substances.

摘要

铜绿假单胞菌是一种广泛存在于环境中的细菌，是人类机会性感染的三大主要病因之一。它作为病原体突出的一个主要因素是其对抗生素和消毒剂的固有抗性。在此，我们报告了铜绿假单胞菌PAO1菌株的完整序列。该序列有630万个碱基对，是已测序的最大细菌基因组，该序列为深入了解铜绿假单胞菌的多功能性和固有耐药性的基础提供了线索。与其更大的基因组大小和环境适应性一致，铜绿假单胞菌拥有细菌基因组中观察到的最高比例的调控基因，以及大量参与有机化合物分解代谢、运输和外排的基因，还有四个潜在的趋化系统。我们认为，铜绿假单胞菌基因组的大小和复杂性反映了一种进化适应，使其能够在多样的环境中茁壮成长，并抵抗各种抗菌物质的影响。