Hu Xi, Tierney Mark T, Grinstaff Mark W
Department of Chemistry, Paul M. Gross Chemical Laboratory, Duke University, Durham, North Carolina 27708, USA.
Bioconjug Chem. 2002 Jan-Feb;13(1):83-9. doi: 10.1021/bc0100509.
A facile procedure for the incorporation of phenothiazine at the terminus of oligodeoxynucleotides is reported. Phenothiazine is covalently linked to the 5'-position of 2'-deoxyadenosine and thymidine. Next, the corresponding phosphoramidites are prepared, and then the labeled nucleosides are incorporated in DNA using an automated DNA solid-phase synthesizer. Phenothiazine labeled oligodeoxynucleotides form stable B-form duplexes with similar melting temperatures, CD spectra, and DSC traces compared to unlabeled DNA duplexes. The favorable photophysical properties of phenothiazine are also retained after covalent attachment to the oligodeoxynucleotide.
报道了一种在寡脱氧核苷酸末端掺入吩噻嗪的简便方法。吩噻嗪与2'-脱氧腺苷和胸苷的5'-位共价连接。接下来，制备相应的亚磷酰胺，然后使用自动DNA固相合成仪将标记的核苷掺入DNA中。与未标记的DNA双链体相比，吩噻嗪标记的寡脱氧核苷酸形成具有相似解链温度、圆二色光谱和差示扫描量热曲线的稳定B型双链体。吩噻嗪与寡脱氧核苷酸共价连接后，其良好的光物理性质也得以保留。
