Yanardağ R, Bolkent S, Kizir A
Department of Chemistry, Faculty of Engineering, Istanbul University, Avcilar, Turkey.
Biol Trace Elem Res. 2001 Dec;83(3):263-73. doi: 10.1385/BTER:83:3:263.
The aim of this study is to investigate whether vitamin E (as DL-alpha-tocopherol acetate) and selenium (as sodium selenate) exert a protective effect against radiation damage. The liver tissue of rats irradiated with a single dose of 1,000 cGy 60Co-gamma-irradiation was examined for morphological changes after the intraperitoneal (ip) administration DL-alpha-tocopherol acetate and sodium selenate as compared to controls. Also, the amounts of blood glutathione and serum alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), and total protein were determined by spectrophotometric methods. Degenerative changes were observed under light and electron microscopy in the liver tissue of the control (radiation only) group. In the group receiving radiation and ip doses of DL-alpha-tocopherol acetate and sodium selenate, the damage to the liver tissue was minimal or absent. In the radiation-only group, a reduction of the blood glutathione level and increases in serum values of AST, ALT, ALP, and LDH activity were observed, whereas in the irradiation-treated group, the reverse was found to occur. Based on these morphological and biochemical observations, it was concluded that the ip administration of DL-alpha-tocopherol acetate and sodium selenate exerts a protective effect against liver radiation damage.
本研究的目的是调查维生素E（以DL-α-生育酚醋酸酯形式）和硒（以硒酸钠形式）是否对辐射损伤具有保护作用。将接受单次1000 cGy 60Co-γ辐射的大鼠的肝脏组织，与对照组相比，在腹腔内（ip）给予DL-α-生育酚醋酸酯和硒酸钠后，检查其形态学变化。此外，通过分光光度法测定血液中谷胱甘肽的含量以及血清丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、碱性磷酸酶（ALP）、乳酸脱氢酶（LDH）和总蛋白的含量。在对照组（仅辐射）的肝脏组织中，在光镜和电镜下均观察到了退行性变化。在接受辐射并腹腔内给予DL-α-生育酚醋酸酯和硒酸钠的组中，肝脏组织的损伤最小或未出现损伤。在仅接受辐射的组中，观察到血液谷胱甘肽水平降低以及血清AST、ALT、ALP和LDH活性升高，而在接受辐射治疗的组中，情况则相反。基于这些形态学和生化观察结果，得出结论：腹腔内给予DL-α-生育酚醋酸酯和硒酸钠对肝脏辐射损伤具有保护作用。
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