Campbell J H, Han C L, Campbell G R
Centre for Research in Vascular Biology, University of Queensland, Brisbane, Australia.
Ann N Y Acad Sci. 2001 Dec;947:18-24; discussion 24-5. doi: 10.1111/j.1749-6632.2001.tb03926.x.
The origin of smooth muscle cells involved in vascular healing was examined. Eighteen C57BL/6 (Ly 5.2) female mice underwent whole body irradiation followed by transfusion with 106 bone nucleated marrow cells from congenic (Ly 5.1) male donors. Successful repopulation by donor marrow was demonstrated after 4 weeks by flow cytometry with FITC-conjugated A20.1/Ly 5.1 monoclonal antibody. The iliac artery of six of the chimeric mice was scratch-injured by five passes of a probe, causing severe medial damage. After 4 weeks the arterial lumen was obliterated by a cell-rich neointima, with alpha-smooth muscle actin-containing cells present around the residual lumen. Approximately half of these cells were of male donor origin, as evidenced by in situ hybridization with a Y chromosome-specific probe. An organized arterial thrombus was formed in the remaining 12 chimeric mice by inserting an 8-0 silk suture into the left common carotid artery. Donor cells staining with alpha-smooth muscle actin were found in those arteries sustaining serious damage but not in arteries with minimal damage. Our results suggest that bone marrow-derived cells are recruited in vascular healing as a complementary source of smooth muscle-like cells when the media is severely damaged and few resident smooth muscle cells are available to effect repair.
对参与血管愈合的平滑肌细胞的起源进行了研究。18只C57BL/6（Ly 5.2）雌性小鼠接受全身照射，随后输注来自同基因（Ly 5.1）雄性供体的106个有核骨髓细胞。4周后，通过使用异硫氰酸荧光素（FITC）偶联的A20.1/Ly 5.1单克隆抗体进行流式细胞术证明供体骨髓成功再填充。6只嵌合小鼠的髂动脉用探针刮擦5次造成损伤，导致严重的中膜损伤。4周后，富含细胞的新生内膜使动脉管腔闭塞，在残余管腔周围存在含α平滑肌肌动蛋白的细胞。用Y染色体特异性探针进行原位杂交证明，这些细胞中约有一半来自雄性供体。通过将8-0丝线缝合线插入左颈总动脉，在其余12只嵌合小鼠中形成有组织的动脉血栓。在那些遭受严重损伤的动脉中发现了用α平滑肌肌动蛋白染色的供体细胞，但在损伤最小的动脉中未发现。我们的结果表明，当血管中膜严重受损且几乎没有驻留的平滑肌细胞可用于修复时，骨髓来源的细胞会被募集到血管愈合过程中，作为平滑肌样细胞的补充来源。
