结核分枝杆菌和鸟分枝杆菌复合群在巨噬细胞内的传代改变了这些微生物的药物敏感性，这是通过使用巨噬细胞和II型肺泡上皮细胞进行细胞内药敏试验确定的。
Intramacrophage passage of Mycobacterium tuberculosis and M. avium complex alters the drug susceptibilities of the organisms as determined by intracellular susceptibility testing using macrophages and type II alveolar epithelial cells.
作者信息
Tomioka Haruaki, Sato Katsumasa, Sano Chiaki, Sano Keisuke, Shimizu Toshiaki
机构信息
Department of Microbiology and Immunology, Shimane Medical University, Izumo, Shimane 693-8501, Japan.
出版信息
Antimicrob Agents Chemother. 2002 Feb;46(2):519-21. doi: 10.1128/AAC.46.2.519-521.2002.
Abstract
Mycobacterium tuberculosis and M. avium complex strains given intramacrophage passage (I-type) were compared with those cultured in a liquid medium (E-type) for their drug susceptibilities when they were replicating in Mono-Mac-6 macrophages or A-549 cells. Their intracellular susceptibilities to rifalazil, clarithromycin, and levofloxacin were decreased more in I-type organisms than in E-type organisms, except that their rifalazil susceptibility inside A-549 cells was markedly increased in I-type organisms.
摘要
将经巨噬细胞内传代培养的结核分枝杆菌和鸟分枝杆菌复合群菌株（I型）与在液体培养基中培养的菌株（E型）进行比较，观察它们在单核巨噬细胞-6或A-549细胞中复制时的药敏情况。I型菌株对利福拉齐、克拉霉素和左氧氟沙星的细胞内药敏性比E型菌株下降得更多，不过I型菌株在A-549细胞内对利福拉齐的药敏性显著增加。
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