膜锚定的刚地弓形虫表面抗原1（SAG1）在原始原生动物十二指肠贾第虫中的正确构象表达。
Conformationally correct expression of membrane-anchored Toxoplasma gondii SAG1 in the primitive protozoan Giardia duodenalis.
作者信息
Marti Matthias, Li Yajie, Köhler Peter, Hehl Adrian B
机构信息
Institute of Parasitology, University of Zürich, CH-8057 Zürich, Switzerland.
出版信息
Infect Immun. 2002 Feb;70(2):1014-6. doi: 10.1128/IAI.70.2.1014-1016.2002.
Abstract
To explore the possibility of expressing membrane-anchored exodomains of heterologous surface antigens in Giardia, a chimeric construct containing the Toxoplasma gondii SAG1 gene was made. The Giardia system is shown here to provide a means of generating correctly folded chimeric surface proteins in a native and unmodified form.
摘要
为了探索在贾第虫中表达异源表面抗原膜锚定胞外结构域的可能性，构建了一个包含刚地弓形虫SAG1基因的嵌合构建体。本文展示了贾第虫系统可提供一种以天然未修饰形式产生正确折叠的嵌合表面蛋白的方法。
相似文献
1
Conformationally correct expression of membrane-anchored Toxoplasma gondii SAG1 in the primitive protozoan Giardia duodenalis.膜锚定的刚地弓形虫表面抗原1（SAG1）在原始原生动物十二指肠贾第虫中的正确构象表达。
Infect Immun. 2002 Feb;70(2):1014-6. doi: 10.1128/IAI.70.2.1014-1016.2002.
2
Expression of in vivo biotinylated recombinant antigens SAG1 and SAG2A from Toxoplasma gondii for improved seroepidemiological bead-based multiplex assays.用于改进基于 bead 的血清流行病学多重检测的弓形虫活体内生物素化重组抗原 SAG1 和 SAG2A 的表达。
BMC Biotechnol. 2020 Oct 6;20(1):53. doi: 10.1186/s12896-020-00646-7.
3
Design and characterization of a recombinant colorimetric SAG1-alkaline phosphatase conjugate to detect specific antibody responses against Toxoplasma gondii.设计和鉴定一种重组比色 SAG1-碱性磷酸酶缀合物，以检测针对弓形虫的特异性抗体反应。
J Immunol Methods. 2013 Aug 30;394(1-2):107-14. doi: 10.1016/j.jim.2013.05.010. Epub 2013 May 28.
4
The Development of Recombinant Trivalent Chimeric Proteins as an Alternative to Lysate Antigen (TLA) in Enzyme-Linked Immunosorbent Assay (ELISA) for the Detection of Immunoglobulin G (IgG) in Small Ruminants.重组三价嵌合蛋白作为酶联免疫吸附测定（ELISA）中裂解物抗原（TLA）的替代物用于检测小反刍动物免疫球蛋白G（IgG）的研究进展
Int J Mol Sci. 2024 Apr 16;25(8):4384. doi: 10.3390/ijms25084384.
5
Crystal structure of the complex between the monomeric form of Toxoplasma gondii surface antigen 1 (SAG1) and a monoclonal antibody that mimics the human immune response.刚地弓形虫表面抗原1（SAG1）单体形式与模拟人类免疫反应的单克隆抗体之间复合物的晶体结构。
J Mol Biol. 2005 Nov 25;354(2):447-58. doi: 10.1016/j.jmb.2005.09.028. Epub 2005 Sep 30.
6
Immunogenic characterization of the chimeric surface antigen 1 and 2 (SAG1/2) of Toxoplasma gondii expressed in the yeast Pichia pastoris.毕赤酵母表达的弓形虫嵌合表面抗原 1 和 2（SAG1/2）的免疫原性特征。
Parasitol Res. 2011 Sep;109(3):871-8. doi: 10.1007/s00436-011-2315-6. Epub 2011 Apr 1.
7
High-level expression and purification of immunogenic recombinant SAG1 (P30) of Toxoplasma gondii in Escherichia coli.
Protein Expr Purif. 2001 Oct;23(1):33-7. doi: 10.1006/prep.2001.1483.
8
Structural and functional characterization of SporoSAG: a SAG2-related surface antigen from Toxoplasma gondii.结构与功能分析 SporoSAG：刚地弓形虫 SAG2 相关表面抗原。
J Biol Chem. 2010 Apr 16;285(16):12063-70. doi: 10.1074/jbc.M109.054866. Epub 2010 Feb 17.
9
Reactivity of synthetic SAG1 (p30) peptide sequences with RH, S273 and Beverley strain-induced anti-Toxoplasma gondii antibodies.合成的SAG1（p30）肽序列与RH、S273和贝弗利株诱导的抗弓形虫抗体的反应性。
Pathobiology. 2007;74(1):50-6. doi: 10.1159/000101051.
10
Serodiagnosis of Toxoplasma gondii infection in cats by enzyme-linked immunosorbent assay using recombinant SAG1.使用重组SAG1通过酶联免疫吸附测定法对猫弓形虫感染进行血清学诊断。
Vet Parasitol. 2001 Dec 3;102(1-2):35-44. doi: 10.1016/s0304-4017(01)00522-2.
引用本文的文献
1
Expression of in vivo biotinylated recombinant antigens SAG1 and SAG2A from Toxoplasma gondii for improved seroepidemiological bead-based multiplex assays.用于改进基于 bead 的血清流行病学多重检测的弓形虫活体内生物素化重组抗原 SAG1 和 SAG2A 的表达。
BMC Biotechnol. 2020 Oct 6;20(1):53. doi: 10.1186/s12896-020-00646-7.
2
Production of the main surface antigen of Toxoplasma gondii in tobacco leaves and analysis of its antigenicity and immunogenicity.弓形虫主要表面抗原在烟草叶片中的表达及其抗原性和免疫原性分析。
Mol Biotechnol. 2005 May;30(1):41-50. doi: 10.1385/MB:30:1:041.
3
The secretory apparatus of an ancient eukaryote: protein sorting to separate export pathways occurs before formation of transient Golgi-like compartments.一种古老真核生物的分泌装置：蛋白质分选以分离输出途径发生在类似高尔基体的瞬时区室形成之前。
Mol Biol Cell. 2003 Apr;14(4):1433-47. doi: 10.1091/mbc.e02-08-0467.
本文引用的文献
1
Characterization of Toxoplasma gondii surface antigen 1 (SAG1) secreted from Pichia pastoris: evidence of hyper O-glycosylation.从毕赤酵母分泌的刚地弓形虫表面抗原1（SAG1）的特性：高O-糖基化的证据。
Biotechnol Appl Biochem. 2001 Feb;33(1):35-45. doi: 10.1042/ba20000069.
2
Glycosylation and palmitoylation are common modifications of giardia variant surface proteins.糖基化和棕榈酰化是贾第虫变异表面蛋白常见的修饰方式。
Mol Biochem Parasitol. 2000 Jun;109(1):61-5. doi: 10.1016/s0166-6851(00)00229-2.
3
Stage-specific expression and targeting of cyst wall protein-green fluorescent protein chimeras in Giardia.贾第虫中囊壁蛋白-绿色荧光蛋白嵌合体的阶段特异性表达及靶向作用
Mol Biol Cell. 2000 May;11(5):1789-800. doi: 10.1091/mbc.11.5.1789.
4
Recombinant antigens to detect Toxoplasma gondii-specific immunoglobulin G and immunoglobulin M in human sera by enzyme immunoassay.通过酶免疫测定法检测人血清中弓形虫特异性免疫球蛋白G和免疫球蛋白M的重组抗原。
J Clin Microbiol. 2000 Mar;38(3):1144-50. doi: 10.1128/JCM.38.3.1144-1150.2000.
5
Baculovirus-directed expression and secretion of a truncated version of Toxoplasma SAG1.杆状病毒介导的截短型弓形虫表面抗原1（Toxoplasma SAG1）的表达与分泌
Mol Biochem Parasitol. 1999 Oct 15;103(2):267-72. doi: 10.1016/s0166-6851(99)00119-x.
6
Analysis of Toxoplasma gondii stably transfected with a transmembrane variant of its major surface protein, SAG1.对稳定转染其主要表面蛋白SAG1跨膜变体的刚地弓形虫的分析。
J Cell Sci. 1998 Jan;111 ( Pt 1):23-9. doi: 10.1242/jcs.111.1.23.
7
The variant-specific surface protein of Giardia, VSP4A1, is a glycosylated and palmitoylated protein.贾第虫的变异特异性表面蛋白VSP4A1是一种糖基化和棕榈酰化蛋白。
Biochem J. 1997 Feb 15;322 ( Pt 1)(Pt 1):49-56. doi: 10.1042/bj3220049.
8
Conformationally appropriate expression of the Toxoplasma antigen SAG1 (p30) in CHO cells.弓形虫抗原SAG1（p30）在CHO细胞中的构象适宜表达。
Infect Immun. 1994 Jan;62(1):203-9. doi: 10.1128/iai.62.1.203-209.1994.
9
Giardia lamblia: post-translational processing and status of exposed cysteine residues in TSA 417, a variable surface antigen.蓝氏贾第鞭毛虫：可变表面抗原TSA 417的翻译后加工及暴露的半胱氨酸残基状态
Exp Parasitol. 1993 Nov;77(3):295-305. doi: 10.1006/expr.1993.1087.
10
Similarities between the primary structures of two distinct major surface proteins of Toxoplasma gondii.
J Biol Chem. 1994 Jun 10;269(23):16217-22.
Zotero 插件