Aubert D, Maine G T, Villena I, Hunt J C, Howard L, Sheu M, Brojanac S, Chovan L E, Nowlan S F, Pinon J M
Laboratoire de Parasitologie, EA2070, IFR 53 CHU Maison Blanche, 51092 REIMS Cédex, France.
J Clin Microbiol. 2000 Mar;38(3):1144-50. doi: 10.1128/JCM.38.3.1144-1150.2000.
We have evaluated the diagnostic utility of eleven Toxoplasma gondii recombinant antigens (P22 [SAG2], P24 [GRA1], P25, P28 [GRA2], P29 [GRA7], P30 [SAG1], P35, P41 [GRA4], P54 [ROP2], P66 [ROP1], and P68) in immunoglobulin G (IgG) and IgM recombinant enzyme-linked immunosorbent assays (Rec-ELISAs). Following an initial evaluation, six recombinant antigens (P29, P30, P35, P54, P66, and P68) were tested in the IgG and IgM Rec-ELISAs with four groups of samples which span the toxoplasmosis disease spectrum (negative, chronic infection, acute infection, and recent seroconversion). Our results suggest that the combination of P29, P30, and P35 in an IgG Rec-ELISA and the combination of P29, P35, and P66 in an IgM Rec-ELISA can replace the tachyzoite antigen in IgG and IgM serologic tests, respectively. The relative sensitivity, specificity, and agreement for the IgG P29-P30-P35 Rec-ELISA were 98.4, 95.7, and 97.2%, respectively. The resolved sensitivity, specificity, and agreement for the IgM P29-P35-P66 Rec-ELISA were 93.1, 95.0, and 94. 5%, respectively. Relative to the tachyzoite-based immunocapture IgM assay, the IgM P29-P35-P66 Rec-ELISA detects fewer samples that contain IgG antibodies with elevated avidity from individuals with an acute toxoplasmosis.
我们评估了11种刚地弓形虫重组抗原（P22 [SAG2]、P24 [GRA1]、P25、P28 [GRA2]、P29 [GRA7]、P30 [SAG1]、P35、P41 [GRA4]、P54 [ROP2]、P66 [ROP1]和P68）在免疫球蛋白G（IgG）和IgM重组酶联免疫吸附测定（Rec-ELISA）中的诊断效用。经过初步评估后，在IgG和IgM Rec-ELISA中用四组涵盖弓形虫病疾病谱的样本（阴性、慢性感染、急性感染和近期血清转化）对6种重组抗原（P29、P30、P35、P54、P66和P68）进行了检测。我们的结果表明，IgG Rec-ELISA中P29、P30和P35的组合以及IgM Rec-ELISA中P29、P35和P66的组合可分别替代IgG和IgM血清学检测中的速殖子抗原。IgG P29-P30-P35 Rec-ELISA的相对敏感性、特异性和一致性分别为98.4%、95.7%和97.2%。IgM P29-P35-P66 Rec-ELISA的分辨敏感性、特异性和一致性分别为93.1%、95.0%和94.5%。相对于基于速殖子的免疫捕获IgM测定，IgM P29-P35-P66 Rec-ELISA检测到的来自急性弓形虫病患者且IgG抗体亲和力升高的样本较少。
