含有人类类风湿关节炎共享表位的犬类主要组织相容性复合体等位基因会使犬类易患类风湿性关节炎。

Dog MHC alleles containing the human RA shared epitope confer susceptibility to canine rheumatoid arthritis.

作者信息

Ollier W E, Kennedy L J, Thomson W, Barnes A N, Bell S C, Bennett D, Angles J M, Innes J F, Carter S D

机构信息

Mammalian Immunogenetics Research Group, Faculty of Veterinary Medicine, University of Liverpool, L69 3BX, UK.

出版信息

Immunogenetics. 2001 Oct;53(8):669-73. doi: 10.1007/s002510100372. Epub 2001 Oct 13.

DOI:10.1007/s002510100372

PMID:11797101

Abstract

To determine whether canine rheumatoid arthritis (CRA) is associated with dog MHC (DLA-DRB1) alleles which contain the QRRAA/RKRAA conserved third hypervariable region (3HVR) sequence, DNA samples were extracted from 61 dogs with clinically diagnosed small-joint polyarthritis and from 425 controls. Breed-matched controls were available for 41 cases. DLA-DRB1 genotypes were identified using molecular typing methods. Phenotype frequencies were compared between cases and controls and odds ratios with 95% confidence intervals calculated. Several DLA-DRB1 alleles were associated with increased risk for CRA: DLA-DRB1002, DRB1009, and DRB1018. This was also observed for the presence of any shared epitope (SE)-bearing allele. The associations with DLA-DRB1002 and the SE were maintained when only breed-matched cases and controls were compared. This study suggests that a conserved amino acid motif in the 3HVR present in some DRB1 alleles of both dogs and humans is associated with rheumatoid arthritis in both species.

摘要

为了确定犬类类风湿性关节炎（CRA）是否与包含QRRAA/RKRAA保守第三高变区（3HVR）序列的犬类主要组织相容性复合体（DLA - DRB1）等位基因相关，从61只临床诊断为小关节多关节炎的犬以及425只对照犬中提取了DNA样本。41例病例有品种匹配的对照。使用分子分型方法鉴定DLA - DRB1基因型。比较病例组和对照组的表型频率，并计算95%置信区间的优势比。几种DLA - DRB1等位基因与CRA风险增加相关：DLA - DRB1002、DRB1009和DRB1018。对于任何携带共享表位（SE）的等位基因的存在也观察到了这种情况。当仅比较品种匹配的病例和对照时，与DLA - DRB1002和SE的关联仍然存在。这项研究表明，犬类和人类某些DRB1等位基因中存在的3HVR中的保守氨基酸基序与两个物种的类风湿性关节炎相关。