Tang Y, Deng C, Du Q, Li G, Zeng Z, Zhang J, Miu S
Sichuan Provincial People's Hospital, Chengdu 610072, China.
Zhonghua Nei Ke Za Zhi. 2001 Apr;40(4):226-8.
To explore whether detecting minimal residual disease (MRD) of leukemia with hypermethylation of the calcitonin gene as molecular genetic marker of leukemic clone may predict the prognosis.
Polymerase chain reaction (PCR) in combination with digestion of DNA with HpaII was used to examine the methylation patterns of the calcitonin gene in 29 cases with acute leukemia and 8 cases with transformation of chronic myeloid leukemia. By using PCR, MRD was longitudinally detected in patients who were positive for hypermethylation of the calcitonin gene as molecular genetic marker.
Twenty patients with acute leukemia and transformation of chronic myeloid leukemia had MRD after complete remission. Bone marrow relapse occurred soon when MRD persisted or reappeared. It may predict bone marrow relapse two to eleven months earlier. The patients who were negative for MRD early and remained persistently negative may acquire prolonged survival.
MRD of leukemia may be monitored by using PCR with hypermethylation of the calcitonin gene as molecular genetic marker for leukemic clone. It may prove useful in predicting the prognosis of leukemia.
探讨以降钙素基因高甲基化作为白血病克隆的分子遗传标记来检测白血病微小残留病（MRD）是否可预测预后。
采用聚合酶链反应（PCR）结合HpaII酶切DNA的方法检测29例急性白血病患者及8例慢性髓性白血病转化患者降钙素基因的甲基化模式。对于降钙素基因高甲基化作为分子遗传标记呈阳性的患者，采用PCR纵向检测其MRD。
20例急性白血病及慢性髓性白血病转化患者完全缓解后存在MRD。当MRD持续存在或再次出现时，很快会发生骨髓复发。它可提前2至11个月预测骨髓复发。早期MRD阴性且持续保持阴性的患者可能获得较长生存期。
可采用以降钙素基因高甲基化作为白血病克隆分子遗传标记的PCR方法监测白血病的MRD。这可能对预测白血病预后有用。
